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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b_Q69L

Plasmid

#186272

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His-tagged at N terminal

Expression

Bacterial

Mutation

Changed Q69 into L

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLNCX myr Akt1

Plasmid

#17245

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

Myr

Expression

Mammalian

Mutation

Constitutively active

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pLV Tmx1-V5

Plasmid

#175138

Purpose

Lentiviral expression of V5-tagged mouse Tmx1

Depositor

Larry Gerace

Insert

Tmx1 (Tmx1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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mouse Drp1(K38A)

Plasmid

#26049

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Drp1 (Dnm1l Mouse)

Expression

Mammalian

Mutation

K38A GTPase mutant

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122

Plasmid

#223184

Purpose

Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Inserts

luciferase (LOC116160065 firefly)
mir122 (Mir122 Mouse)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Mo-Trip6

Plasmid

#27254

Depositor

Thomas Gilmore

Article

Wang et al Gene. 1999 Jul 8. 234(2):403-9.

Insert

Trip6 (Trip6 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 FLAG

Plasmid

#17521

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Blast Twist ER

Plasmid

#18799

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

twist (Twist1 Mouse)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Snapin

Plasmid

#118742

Purpose

Expresses EGFP tagged Snapin

Depositor

Beverly Koller

Insert

Snapin cDNA (Snapin Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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2.1 kB VEGF-luc

Plasmid

#27988

Depositor

Patricia D'Amore

Article

Loureiro et al Biochem Biophys Res Commun. 2005 Jan 14.

Insert

2.1 kB VEGF

Use

Luciferase

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCI-neo-Flag-DHHC5

Plasmid

#85812

Purpose

To rescue the expression of DHHC5 in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-CAF1-C-10

Plasmid

#231782

Purpose

Visualization of nucleus in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-CAF1-C-10 (Chaf1a Mouse)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Syt7 KI

Plasmid

#131488

Purpose

Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Syt7, Syt7 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Nlgn3 KI

Plasmid

#131501

Purpose

Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Nlgn3, Nlgn3 Mouse, Rat)

Use

CRISPR

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-mGLIS1

Plasmid

#33273

Purpose

Retroviral expression of mouse Glis1

Depositor

Shinya Yamanaka

Article

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1, Gateway casette A (Glis1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt7

Plasmid

#184508

Purpose

Mammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 7 (Syt7 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CHOP::GFP

Plasmid

#21898

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

CHOP (Ddit3 Mouse)

Use

Non-viral

Tags

EGFP

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

Plasmid

#183252

Purpose

Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

anti-CD19 scFvFc (FMC-63)

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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