We narrowed to 10,928 results for: cat.1

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pGEX-4T Doc2beta C2AB(125) H158C

Plasmid

#134691

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

H158C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) F222C

Plasmid

#134692

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

F222C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) A298C

Plasmid

#134693

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

A298C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R459E

Plasmid

#164024

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R459E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R464E

Plasmid

#164025

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E485R

Plasmid

#164026

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E485R

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E490R

Plasmid

#164027

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E490R

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R495E

Plasmid

#164028

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R495E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C D511K

Plasmid

#164029

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, D511K

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C Y527A

Plasmid

#164030

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, Y527A

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Progerin

Plasmid

#118710

Purpose

Lentiviral TET-ON inducible GFP-Progerin

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-LIC1 G domain

Plasmid

#74602

Purpose

expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHnS KI;Actb Donor;3xV5 KO;Dlg4

Plasmid

#240292

Purpose

KI:Actb Donor:3xV5 KO:Dlg4

Depositor

Yuki Ogawa

Insert

KI gRNA for Actb

Use

AAV

Mutation

NA

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pZNF253.1.0-gDNA

Plasmid

#132461

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

ZNF253 (ZNF253 Human)

Use

CRISPR

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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E2F1 in pGEX4t

Plasmid

#112976

Purpose

For protein expression and purification of Drosophila E2F1

Depositor

Terry Orr-Weaver

Insert

E2F1 (E2f1 Fly)

Expression

Bacterial

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-1α-Venus-Nup107-FRT

Plasmid

#247344

Purpose

Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

Nup107 (NUP107) (NUP107 Human)

Tags

Venus

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTU2-D-lacZ

Plasmid

#72953

Purpose

Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - D site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU2-D-RFP

Plasmid

#72957

Purpose

Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - D site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSLR

Plasmid

#51500

Purpose

Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Plant t-dna plasmid

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-MHCK7-R-GECO

Plasmid

#109374

Purpose

Expresses calcium indicator R-GECO in muscle cells

Depositor

Nenad Bursac

Article

Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

Insert

R-GECO

Use

Lentiviral

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2834

Plasmid

#160673

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
sspB-mSc-sspB

Plasmid

#114422

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2774

Plasmid

#160667

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2836

Plasmid

#160674

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0245

Plasmid

#162284

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
2xsspB-mSc-sspB

Plasmid

#114423

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC0CM0258

Plasmid

#162291

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
U6.3>gRNA.f+e

Plasmid

#99139

Purpose

Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

gRNA

Use

CRISPR

Promoter

chicken U6.3

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-ArcLight-ST

Plasmid

#182318

Purpose

Fluorescent voltage indicator with left-shifted voltage-dependency

Depositor

Rafael Yuste

Article

Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

Insert

ArcLight-ST

Expression

Mammalian

Mutation

Mutation in an amino acid Q217E of ArcLight-MT

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Fast-GCaMP6f-RS09

Plasmid

#67160

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS09

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

L414T within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Fast-GCaMP6f-RS06

Plasmid

#67159

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS06

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

M374Q within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0285

Plasmid

#162290

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CB0246

Plasmid

#162286

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0549

Plasmid

#162300

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, SUMO-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0283

Plasmid

#162288

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 10,928 results for: cat.1