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pFA6a-hygML

Plasmid

#73892

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

hygML

Expression

Bacterial and Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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369 pTol-lmo2:egfpDest

Plasmid

#73488

Purpose

Tol2 vector with lmo2 promoter upstream of EGFP, attR1/R2 cassette and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

lmo2 promoter:EGFP-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3c2-PK3

Plasmid

#78390

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
natCX

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRB33-GFP

Plasmid

#165483

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-GFP-TEV

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSGE_91_4xUAS_dCP

Plasmid

#71169

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

91_4xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3b-PK9

Plasmid

#78363

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
hygMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pQYD

Plasmid

#48104

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

SYFP2

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3C06

Plasmid

#70979

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG9246

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3D11

Plasmid

#71007

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Acf1

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pYPQ132D-RZ-Lb

Plasmid

#134348

Purpose

Golden Gate entry vector; empty vector to clone the 2nd LbCas12a crRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-M

Plasmid

#1128

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

M (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pStA0::eyfp

Plasmid

#114351

Purpose

efyp in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

efyp (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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Gal ts-lux

Plasmid

#1329

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

bacterial luciferase-V.h.MAV

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGEX Shark-tSH2

Plasmid

#126672

Purpose

Expression of Shark tandem SH2 domains SNAP tagged for use as FRET reporter

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark tandem SH2 domains

Expression

Bacterial

Available Since

July 12, 2020

Availability

Academic Institutions and Nonprofits only

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pZHY402

Plasmid

#45044

Depositor

Daniel Voytas

Article

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

TALEN T30 Target Sequence

Use

Reporter; plant expression

Promoter

35S

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pNXRVd-HACre

Plasmid

#78404

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
ura4+ (ura4 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3b2-HA3

Plasmid

#78387

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
hygML

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3c2-FL3

Plasmid

#78391

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
natCX

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3c2-Myc13

Plasmid

#78393

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

13xMyc
natCX

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3b-PK12

Plasmid

#78364

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

12xPK
hygMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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