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We narrowed to 7,755 results for: Tet-on

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Showing: 2081 - 2100 of 7755 results

  2. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

    Plasmid
    #231595
    Purpose
    Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter
    Depositor
    Marvin Tanenbaum
    Article
    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.
    Insert
    Kozak sequence-Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
    Expression
    Mammalian
    Available Since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    231595-plasmid-map-sequence-id-459554

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  4. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  5. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Article
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    TRE and U6
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

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  6. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid
    #154827
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    TagBFP
    HygR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154827-plasmid-map-sequence-id-306202

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  9. pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199366
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    3C-SUMOEu-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199366-plasmid-map-sequence-id-389910

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  10. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82704-plasmid-map-sequence-id-158051

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  14. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc

    Plasmid
    #193347
    Purpose
    tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc)
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2, Pou5f1 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Sox2A61V is a highly cooperative point mutant of …
    Promoter
    TetO mini CMV
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193347-plasmid-map-sequence-id-379820

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  15. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

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  18. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

    Plasmid
    #199355
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    TagBFP-P2A-ALFA-SUMOstar
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199355-plasmid-map-sequence-id-389902

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Showing: 2081 - 2100 of 7755 results