We narrowed to 7,472 results for: lif

1. Sema3g.a(L)-Fc-His

   Plasmid

   #72151

   Purpose

   Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3g.a (Sema3g Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SnFR-gamma2

   Plasmid

   #165495

   Purpose

   Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

   Depositor

   Andrew Plested

   Article

   Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

   Insert

   iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-mCherry-SPASTIN

   Plasmid

   #180647

   Purpose

   Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Starts at M87, has siRNA resistance to GAACAGUGUG…

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHalo-MyosinIIB-C25

   Plasmid

   #128574

   Purpose

   Studies of cellular contractility.

   Depositor

   Dylan Burnette

   Article

   Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

   Insert

   Myosin IIB (MYH10 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-Lck-FRB-mTurquoise2

   Plasmid

   #176130

   Purpose

   Plasma membrane localized FRB, rapamycin heterodimerization

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   FRB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. UAS-u(S)Cas9

   Plasmid

   #127383

   Purpose

   Expresses Cas9 at high level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(S)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-mCherry-mTrim21ΔRING-Box

   Plasmid

   #105523

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Expression

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sema3e(L)-AP-His

   Plasmid

   #72019

   Purpose

   Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3e (Sema3e Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-uTEV1-NES

   Plasmid

   #171006

   Purpose

   HiLITR protease with C-terminal NNES (Cytoplasmic)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-NES
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-shLacZ-1735

    Plasmid

    #115454

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shLacZ-1735
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Plxna4-AP-His

    Plasmid

    #71999

    Purpose

    Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna4 (Plxna4 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRES_EGFP_GA_KCNE1

    Plasmid

    #173160

    Purpose

    For mammalian expression of KCNE1 and creating variants

    Depositor

    Al George

    Article

    Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

    Insert

    KCNE1 (KCNE1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-RNF68

    Plasmid

    #126545

    Purpose

    Expresses mCherry-tagged RNF68 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid

    #127359

    Purpose

    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CVS-N2c-tdTomato-iCre

    Plasmid

    #172374

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    tdTomato + iCre
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-HA-RNF68

    Plasmid

    #126548

    Purpose

    Expresses FLAG-HA-tagged RNF68 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. All-in-one split-ADAR2(E488Q, N496F)

    Plasmid

    #170148

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-FKBP-SAC1-HLx8

    Plasmid

    #108136

    Purpose

    Recruitable SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMHE-mEGFP-mTrim21ΔRING-Box

    Plasmid

    #105520

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sema6c-Fc-His

    Plasmid

    #72167

    Purpose

    Expresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6c (Sema6c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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