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pRSCgfp-hAIM2

Plasmid

#51666

Purpose

Express GFP and hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Inserts

AIM2 (AIM2 Human)
GFP

Expression

Mammalian

Promoter

CMV and RSV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pJBL724

Plasmid

#140394

Purpose

Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-hucO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-His6

Plasmid

#51536

Purpose

Express His6-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJBL726

Plasmid

#140396

Purpose

Expresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-smtO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDI

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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CBP-HAT-pET

Plasmid

#21091

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Tags

His and T7 tag

Expression

Bacterial

Mutation

HAT domain aa 1171-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
PEP113E

Plasmid

#97399

Purpose

binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Peroxisome target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ

Plasmid

#192387

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PGL3-MK17

Plasmid

#21486

Depositor

Shoumo Bhattacharya

Article

Leung et al Genomics. 1999 Nov 1. 61(3):307-13.

Insert

Cited2 Promoter (CITED2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)

Plasmid

#191179

Purpose

Negative control sensor in human cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s2:EGFP (negative control)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CP1300-ABEmax

Plasmid

#119810

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(22/19), AB(monomer), wt

Plasmid

#22380

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His and myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p3003 pGEM E6-AP

Plasmid

#8644

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pU6nH1-HD10kb

Plasmid

#172657

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD10kbA/pegRNA-HD10kbB

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-T7-hAIM2

Plasmid

#51541

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GFP and T7

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

Plasmid

#191151

Purpose

Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L72:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

Plasmid

#192412

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

Plasmid

#192413

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-APC11

Plasmid

#19898

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

APC11 (ANAPC11 Human)

Tags

HA and T7

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
HOXB6-pCMV

Plasmid

#21258

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

HOXB6 (HOXB6 Human)

Tags

FLAG_Pro Tag

Expression

Bacterial and Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pMX156

Plasmid

#29482

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-beta (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(26/19) AB(monomer), wt

Plasmid

#22381

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-deltaPYD

Plasmid

#51535

Purpose

Express His6-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191184

Purpose

Expression of RPLP1 sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:RPLP1.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

Plasmid

#191175

Purpose

Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (frameshifted)

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192396

Purpose

To act as a 2-input state of the AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192391

Purpose

To act as a 2-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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F5A deltaCT S6K1 MSCV puro

Plasmid

#8464

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta ctp70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids mutation …

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pMX155

Plasmid

#29481

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-alpha (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

E140K

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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