Addgene

We narrowed to 11,638 results for: pcdna

Showing: 3151 - 3180 of 11638 results

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pcDNA3 HRPT2 136X

Plasmid

#11049

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 136X (CDC73 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-gap16-ClopHensor

Plasmid

#26006

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

gap16-ClopHensor

Use

Tags

Gap16 and Strep-II

Expression

Mammalian

Mutation

N-term (16AA) of Gap-43

Promoter

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDDB2-T338M-V5

Plasmid

#167470

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

T338M

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1 K137R

Plasmid

#85694

Purpose

Mammalian expression of mouse GATA1 with K137R mutation

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

K137R (SUMO-1 attachment site)

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CCND1(T286A)

Plasmid

#172631

Purpose

Expresses untagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB2b

Plasmid

#19470

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB2b (DNAJB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp15-HF

Plasmid

#157722

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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a4 ecto pcDNA3.1 hygro(-)

Plasmid

#113406

Purpose

expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

Insert

human alpha 4 ectodomain

Use

Tags

Strep II tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2.2-IgG1

Plasmid

#149667

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Q842gp120-8his

Plasmid

#100932

Purpose

Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Use

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28BdelN70

Plasmid

#51374

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28B (LIN28B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 70 amino acids deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-hDDB2-K244E-V5

Plasmid

#167471

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

K244E

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA2-DC+Nzf

Plasmid

#1293

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
XE212 RGS9-pcDNA3.1

Plasmid

#16676

Depositor

Randall Moon

Insert

RGS9 (Rgs9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2v4-Fc(IgG1)

Plasmid

#145167

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 4

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 K55R

Plasmid

#78807

Purpose

To overexpress RIPK3 K55R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K55R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 S199A

Plasmid

#78806

Purpose

To overexpress RIPK3 S199A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S199A

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 L198A

Plasmid

#8975

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

L198A

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
Kohinoor-Golgi/pcDNA3

Plasmid

#67779

Purpose

Golgi-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

GT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-UBXD5-Strep-HA

Plasmid

#113493

Purpose

Expression of human UBXD5 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD5 (UBXN11 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(K8,9A)-mPAGFP

Plasmid

#168493

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 Antisense

Plasmid

#60632

Purpose

Negative Control for the pcDNA3.1(+) ZKSCAN1 Sense plasmid

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPHB4 Antisense

Plasmid

#60640

Purpose

Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Ephrin type B receptor 4 (EPHB4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v5-Fc(IgG1)

Plasmid

#145168

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 5

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-α-COPI-WD40

Plasmid

#180758

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Leu181Lys, Leu…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-E2F4CA-Myc

Plasmid

#80428

Purpose

Mammalian expression of constitutively active chick E2F4

Depositor

José María Frade

Article

Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

Insert

E2F transcription factor 4 (E2F4 Chicken)

Use

Tags

Myc

Expression

Mammalian

Mutation

T261E, T263E

Promoter

CMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE2

Plasmid

#120396

Purpose

Expresses HF2-BE2 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE2

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-PKB-pcDNA3-K179A

Plasmid

#24652

Depositor

Jim Woodgett

Insert

PKB (AKT1 )

Use

Tags

HA

Expression

Mammalian

Mutation

K179A

Promoter

Available since

June 1, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L64P

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only