We narrowed to 13,736 results for: sequence
-
Plasmid#148148PurposeMammalian Expression of Hs4EBP3-Y40AL45ADepositorInsertHs4EBP3-Y40AL45A (EIF4EBP3 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3-YLLVtoAAAA_Z
Plasmid#148149PurposeMammalian Expression of Hs4EBP3-YLLVtoAAAADepositorInsertHs4EBP3-YLLVtoAAAA (EIF4EBP3 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1-L75AV81A_Z
Plasmid#148144PurposeMammalian Expression of Hs4EBP1-L75AV81ADepositorInsertHs4EBP1-L75AV81A (EIF4EBP1 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1-Y54AL59A_Z
Plasmid#148145PurposeMammalian Expression of Hs4EBP1-Y54AL59ADepositorInsertHs4EBP1-Y54AL59A (EIF4EBP1 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1-YLLVtoAAAA_Z
Plasmid#148146PurposeMammalian Expression of Hs4EBP1-YLLVtoAAAADepositorInsertHs4EBP1-YLLVtoAAAA (EIF4EBP1 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-Co-RHD
Plasmid#180207PurposeExpresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeastDepositorInsertCo-RHD
TagsGAL4 (1-147) fusionExpressionYeastMutationaa 2-581PromoterAdhIAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182682PurposeContains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.DepositorInsertmouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterNAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor
Plasmid#182686PurposeContains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.DepositorInsertmouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterNAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182685PurposeEncodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.DepositorInsertsExpressionMammalianPromoterCMV and NAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF
Plasmid#148686PurposeBacterial Expression of HsGIGYF2_35-105-E46AE47FN91ADepositorInsertHsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)
ExpressionBacterialAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AE
Plasmid#148575PurposeMammalian Expression of HsGIGYF1_1-177-YYMLtoAAAADepositorInsertHsGIGYF1_1-177-YYMLtoAAAA (GIGYF1 Human)
ExpressionMammalianAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-Hs4EHP_52-234opt_AE
Plasmid#148564PurposeBacterial Expression of Hs4EHP_52-234optDepositorInsertHs4EHP_52-234opt (EIF4E2 Human)
ExpressionBacterialAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-Co-Cterm
Plasmid#180208PurposeExpresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeastDepositorInsertCo-RHD
TagsGAL4 (1-147) fusionExpressionYeastMutationaa 542-1224PromoterAdhIAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PARP2
Plasmid#175810PurposepENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tagsDepositorAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD 5'UTR:YFP:Tnos (GB1483)
Plasmid#160557PurposePart 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)DepositorInsert5'UTR:YFP:Tnos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)
Plasmid#160581PurposePart 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminatorDepositorInsertLuc
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNA sCRNA 2.0 (GB2461)
Plasmid#160593PurposeVersion of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.DepositorInsertsgRNA sCRNA 2.0
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ir7f-T2A-QF2 HDR plasmid
Plasmid#140942PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f geneDepositorInsertIr7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
Mutation4 point mutations (annotated on plasmid map) were…Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUL33
Plasmid#135103PurposeCodon-optimized HSV-1 UL33 with N-terminal HAStrep tagDepositorInsertHSV-1 UL33 codon optimized
TagsHA-Strep-StrepExpressionMammalianAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUL50-CStHA
Plasmid#135105PurposeCodon-optimized HSV-1 UL50 with C-terminal StrepHA tagDepositorInsertHSV-1 UL50 codon optimized
TagsStrep-Strep-HAExpressionMammalianAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUL33-CStHA
Plasmid#135106PurposeCodon-optimized HSV-1 UL33 with C-terminal StrepHA tagDepositorInsertHSV-1 UL33 codon optimized
TagsStrep-Strep-HAExpressionMammalianAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUS2-CStHA
Plasmid#135107PurposeCodon-optimized HSV-1 US2 with C-terminal StrepHA tagDepositorInsertHSV-1 US2 codon optimized
TagsStrep-Strep-HAExpressionMammalianAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Glu39Ala_P2A_Hygro_Barcode
Plasmid#120512PurposeBarcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Glu39Ala (MYC Human)
UseLentiviralMutationPoint mutation changing Glutamic Acid to Alanine …PromoterEF1aAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF8_P2A_Hygro_Barcode
Plasmid#120494PurposeBarcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Neo_WT_ZFP64_CR
Plasmid#118694PurposeExpress sgZFP64e2 resistant cDNADepositorInsertZFP64 (ZFP64 Human)
UseLentiviralMutationsgZFP64e2 targeting site was synonymous mutatedPromoterEF1aAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Neo_ZFP64_N_3XFLAG
Plasmid#118700PurposeExpress 3xflag tagged ZFP64DepositorInsertZFP64 (ZFP64 Human)
UseLentiviralTags3XFLAG was fused to the N terminal of ZFP64 cDNAPromoterEF1AAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H
Plasmid#108374PurposeExpresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertExpressionMammalianPromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-1xTS
Plasmid#109418PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only