We narrowed to 59,329 results for: cloning

1. pREXNH3

   Plasmid

   #47079

   Purpose

   FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Lactococcus lactis expression

   Tags

   3C protease site (PreScission site) and decaHis (…

   Promoter

   NIS (nisin)

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgPal1 (p2Tol-U6-sgPal1-HygR)

   Plasmid

   #71483

   Purpose

   Expresses palindromic sgRNA for use in self-cloning CRISPR

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Insert

   sgPal1
   

   Promoter

   U6

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDXNSM3

   Plasmid

   #49028

   Purpose

   FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site), MYC, and str…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hs.HRAS Q61K

   Plasmid

   #83187

   Purpose

   Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61K mutation

   Depositor

   Dominic Esposito

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   Q61K

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hs.HRAS G13D

   Plasmid

   #83185

   Purpose

   Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G13D mutation

   Depositor

   Dominic Esposito

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G13D

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

   Plasmid

   #49313

   Purpose

   Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Insert

   nls-mCherry-Flag
   

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGC1853

   Plasmid

   #44619

   Depositor

   Robert Oshima

   Article

   Kulesh et al Mol Cell Biol. 1988 Apr;8(4):1540-50.

   Insert

   keratin 18 gene (KRT18 Human)
   

   Use

   Cloning

   Available Since

   June 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-686 (attR4-attR2)

   Plasmid

   #161889

   Purpose

   Multisite Gateway (attR4-attR2) vector for lentivirus production

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest-305 (attR4-attR2)

   Plasmid

   #161895

   Purpose

   Multisite Gateway (attR4-attR2) vector for mammalian protein expression

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hs.HRAS G12C

    Plasmid

    #83182

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12C mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12C

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYEXC3H

    Plasmid

    #49026

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYEXNHG3

    Plasmid

    #49025

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGD008

    Plasmid

    #48838

    Purpose

    Provides an ER retrieval signal as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    ER retrieval signal
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rabbit SERCA2b (pBSII-SK-)

    Plasmid

    #75186

    Purpose

    Cloning vector for ATP2A2

    Depositor

    Jonathan Lytton, David MacLennan

    Article

    Lytton et al J Biol Chem. 1989 Apr 25;264(12):7059-65

    Insert

    ATP2A2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGB002

    Plasmid

    #48820

    Purpose

    Provides the omega element as GreenGate N-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    Omega element
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MXS_H2B

    Plasmid

    #62432

    Purpose

    MXS_chaining vector with H2B

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Histone 2B with leading Kozak sequence
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGGEV_2_+nls-eGFP-HA-_+1_BK+

    Plasmid

    #49312

    Purpose

    Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Insert

    nls-EGFP-HA
    

    Use

    Cloning vector

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGH000

    Plasmid

    #48862

    Purpose

    Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag + terminator modules.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBXNAC3H

    Plasmid

    #47074

    Purpose

    FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), Avi-tag, and…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_MCS3

    Plasmid

    #62397

    Purpose

    MXS_chaining vector with MCS3

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    MluI-XhoI-HindIII-PstI-SalI MCS
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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