We narrowed to 4,296 results for: p2a
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Plasmid#220608PurposeNeuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.DepositorInsert3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
UseAAV and Cre/LoxTags3x HA; ArgiNLSExpressionMammalianPromoterhSynAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171595PurposeWeak human ACE2 abundance from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…ExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118157PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS
Plasmid#199352PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-3CExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP
Plasmid#199364PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-ALFA-P2A-TagBFPExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)
Plasmid#169336PurposeExpression of the LT-Fucci(SA) in mammalian cellsDepositorTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187949PurposeSMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertSMASh-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX
Plasmid#202718PurposeMammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imagingDepositorArticleInsertsPink Flamindo
paGFP-bPAC-CAAX
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA
Plasmid#191685PurposeFor knocking p2a-tdTomato into CLSTN2 locusDepositorInsertCLSTN2 homology arms and p2a-tdTomato
ExpressionMammalianMutationWTAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA
Plasmid#191690PurposeFor knocking p2a-EGFP into OTX2 locusDepositorInsertOTX2 homology arms and p2a-EGFP
ExpressionMammalianMutationWTAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RA
Plasmid#191683PurposeFor knocking p2a-tdTomato into LMX1A locusDepositorInsertLMX1A homology arms and p2a-tdTomato
ExpressionMammalianMutationWTAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118161PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1335 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295S]
Plasmid#107848PurposeTo test the effect of sequence on TDP43 splicing activityDepositorAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PL452-EN1-LA-p2a-mNeonGreen-hGHpA-PGK-neoR-EN1-RA
Plasmid#191684PurposeFor knocking p2a-mNeonGreen into EN1 locusDepositorInsertEN1 homology arms and p2a-mNeonGreen
ExpressionMammalianMutationWTAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA
Plasmid#191688PurposeFor knocking p2a-EFGP into CLSTN2 locusDepositorInsertCLSTN2 homology arms and p2a-EGFP
ExpressionMammalianMutationWTAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V]
Plasmid#107852PurposeTo test the effect of sequence on TDP43 splicing activityDepositorAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1398 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W]
Plasmid#118811PurposeTo test the effect of sequence on TDP43 splicing activityDepositorAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309F]
Plasmid#107851PurposeTo test the effect of sequence on TDP43 splicing activityDepositorAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]
Plasmid#107845PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll hydrophobics combined in four clusters in spl…Available SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]
Plasmid#107844PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll hydrophobics combined in two clusters in spli…Available SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only