We narrowed to 5,328 results for: pcdna3.1

1. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

   Plasmid

   #166798

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   LL100EE

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

   Plasmid

   #158604

   Purpose

   Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

   Plasmid

   #187994

   Purpose

   Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   mucin2 D1D2D'D3 (MUC2 Human)
   

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

   Plasmid

   #62977

   Purpose

   Expresses myc-tagged mouse βTrCP residues 241-569

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   BTRC (Btrc Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   codes for residues 241-569

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

   Plasmid

   #49065

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

   Plasmid

   #168189

   Purpose

   HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
   

   Tags

   HA-Tag

   Expression

   Mammalian

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

   Plasmid

   #166801

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   S730A

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

   Plasmid

   #69903

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

   Plasmid

   #49063

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid

    #126444

    Purpose

    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated

    Depositor

    Jeremy Wilusz

    Article

    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

    Insert

    Fndc3b (Fndc3b Mouse)
    

    Expression

    Mammalian

    Mutation

    circFndc3b exons are separated by a miniature int…

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-MUC2 D1D2D' with cleavable His tag

    Plasmid

    #187993

    Purpose

    Expresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Mucin2 D1D2D' (MUC2 Human)
    

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. XE252 pCDNA3.1(zeo)-hDsh3-333-716

    Plasmid

    #16757

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-333-716-Dsh3 (DVL3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 333-716

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X

    Plasmid

    #16759

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    VSV-KLHL12-Q405X (KLHL12 Human)
    

    Tags

    vsv

    Expression

    Mammalian

    Mutation

    Q405STOP

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG

    Plasmid

    #29583

    Depositor

    Anna Huttenlocher

    Article

    Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

    Insert

    phosphatidylinositol phosphate kinase type I gamma
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Feb. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

    Plasmid

    #111459

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523), C643A

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

    Plasmid

    #69907

    Purpose

    Expresses a circular RNA containing the 5' half of GFP in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    GFP, ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. (363) pcDNA3.1-Nup62-Ubc-Flag-EPEA

    Plasmid

    #168098

    Purpose

    Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Tags

    EPEA-tag, Flag-tag, and Ubc-Tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/cmv

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. XE254 pCDNA3.1(zeo)-hDsh3-497-716

    Plasmid

    #16755

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-497-716-Dsh3 (DVL3 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa 497-716

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127316

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127318

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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