We narrowed to 9,067 results for: sgRNA
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Plasmid#217015PurposeS. aureus sgRNAs backbone along with mCherry expressionDepositorInsertmCherry
UseAAV and CRISPRExpressionMammalianPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSCBE3-NG-HF1-Hypa
Plasmid#218162PurposeThis plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsscbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionExpressionBacterialMutationMutations in the portion of SpCas9 : D10A / N497A…PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9
Plasmid#136660PurposeExpresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSt1Cas9 LMD-9
sgRNA for St1Cas9
ABE7.10
UseCRISPR and Synthetic BiologyTagsSV40 NLS and hSt1Cas9ExpressionMammalianPromoterCAG and U6Available sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV C-terminus ABE8e-V106W targeting Pex1-G844D
Plasmid#247655PurposeAAV genome encoding the C-terminus of ABE8e-V106W editor and Sp sgRNA cassetteDepositorInsertC-terminus ABE8e-V106W editor and Pex1-G844D-targeting sgRNA
UseAAV and CRISPRPromoterCBhAvailable sinceApril 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
AAV N-terminus ABE8e-V106W targeting Pex1-G844D
Plasmid#247654PurposeAAV genome encoding the N-terminus of ABE8e-V106W editor and Sp sgRNA cassetteDepositorInsertN-terminus ABE8e-V106W editor and Pex1-G844D-targeting sgRNA
UseAAV and CRISPRPromoterCBhAvailable sinceApril 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFH99
Plasmid#128204Purposeclassic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertclassic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH100
Plasmid#128205Purposeimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a
Plasmid#233079PurposeExpression vector for sgRNA-S1b and eCas12f1aDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianPromoterhU6, CBhAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3XNeon-nls
Plasmid#75386PurposePCP-3XNeon-nlsDepositorInsertPCP
UseLentiviralTags3XmNeonGreenExpressionMammalianPromoterEFSAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcU6_3 MS2 sgRNA
Plasmid#92394PurposeChick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertchick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
UseCRISPRExpressionMammalianPromoterchick U6.3Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_CELF2
Plasmid#106102PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2DepositorInsertgRNA targeting CELF2 (CELF2 Human)
UseCRISPRAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_TIAL1
Plasmid#106096PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA TIAL1
UseCRISPRAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-A
Plasmid#177784PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
act-AsCpf1
Plasmid#73917Purposeexpression plasmid of AsCpf1DepositorInserthAsCpf1
TagsNLS-3xHAExpressionInsectPromoteract5CAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-MCP-3XBFPnls
Plasmid#75383PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-Puro-P2A-EGFP-PJY463
Plasmid#254300PurposeCloning backbone for guide RNAs compatible with 10x FLEX and CROPseqDepositorInsertSFFV-Puro-P2A-EGFP-WPRE-hU6-sgRNA
UseCRISPR and LentiviralPromoterSFFVAvailable sinceJune 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2
Plasmid#204852PurposeAAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouseDepositorInsertminiSdd6-nSaCas9KKH(D10A)-UGI
UseAAV and CRISPRMutationD10A, E782K, N968K, R1015H in SaCas9PromoterEFS, miniU6Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgMUC4
Plasmid#198399PurposeExpresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMUC4 sgRNA (MUC4 Human)
ExpressionMammalianPromoterU6Available sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
ExpressionBacterialPromoterEc-TTL-P112 and BBa_J23116Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only