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Plasmid#128839PurposeRNA isolation from keratinocytes via TRAPDepositorInsertEGFP-L10a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Pcdh19-GFP
Plasmid#35675DepositorInsertPcdh19 (pcdh19 Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
P1
Plasmid#87089PurposeThis is the P1 part of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmidDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterBcl-2 PromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXcX-PRS-EGFP
Plasmid#25865DepositorInsertPRS driven enhanced green fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-CREM4
Plasmid#106459PurposeCMV promoter with 4 CRE sites mutated, can be used to create a reporter vectorDepositorInsert4 CRE sites mutated
UseTagsExpressionMammalianMutation4 CRE sites mutatedPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LacI-KRAB
Plasmid#232638Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressorDepositorInsertHaloTag-LacI-KRAB
UseTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mGFP in pRS315
Plasmid#221158PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmGFPExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mScarletI in pRS315
Plasmid#221159PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmScarletIExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mNeonGreen in pRS315
Plasmid#221160PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmNeonGreenExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-KO
Plasmid#196489PurposeTo KO Gfap in mouse astrocytesDepositorInsertgRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-melHis10TEV
Plasmid#159428Purposeempty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sitesDepositorTypeEmpty backboneUseTagsmelittin signal sequence (removed in vivo by sign…ExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gfaABC1D-mCherry-CAAX
Plasmid#218189PurposeTo over express mCherry-CAAX under gfaABC1D promoter in astrocytesDepositorInsertmCherry-CAAX
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:GCaMP6s; cryaa:mCherry
Plasmid#230060PurposeExpression of genetically encoded calcium indicator in zebrafish hepatocytes.DepositorInsertGCaMP6s
UseZebrafish expressionTagsExpressionMutationPromoterfabp10aAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLSLR
Plasmid#51500PurposeSingle component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.DepositorTypeEmpty backboneUsePlant t-dna plasmidTagsExpressionMutationPromoterAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP
Plasmid#51494PurposepLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmidDepositorInsertsGFP
us:NPTII repair template
UsePlant t-dna plasmidTagsNLS and flagExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
H2B-SNAPf-mTurquoise2
Plasmid#197494PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the nucleus.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNAPf-mTurquoise2
Plasmid#197495PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the whole cell.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOM20-SNAPf-mTurquoise2
Plasmid#197496PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the mitochondria.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mutLuc
Plasmid#106458Purposeluciferase reporter, CMV promoter (3 NFkB sites mutated)DepositorInsertluciferase reporter, CMV promoter (3 NFkB sites mutated)
UseLuciferaseTagsExpressionMammalianMutation3 NFkB sites mutatedPromoterAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBPcyto-TEV-BseRI
Plasmid#157739Purposeempty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + MBP (cytoplasmic expression; no signal se…ExpressionBacterialMutationPromoterT7Available sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only