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Lenti_CRISPRi_sgAR1

Plasmid

#167000

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 1 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAct-dCBEevoCDA1

Plasmid

#195515

Purpose

Ubiquitous expression of dCBEevoCDA1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoCDA1

Use

CRISPR

Expression

Insect

Promoter

act5C

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)DsRed

Plasmid

#200114

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence, no other pr…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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AV44_pCAG.Cas9-D10A.gRNA.S1

Plasmid

#199258

Purpose

Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9-D10A nickase
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS Donor mClover-LMNA

Plasmid

#98970

Purpose

Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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hCas9_D10A

Plasmid

#41816

Purpose

Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9_D10A

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized, D10A nickase

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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10279-M28-663

Plasmid

#225662

Purpose

Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE60

Plasmid

#90405

Purpose

STAT5 - Stat5 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT5

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBS U3-hTR-500

Plasmid

#28170

Depositor

Kathleen Collins

Article

Fu et al Mol Cell. 2003 May . 11(5):1361-72.

Insert

U3-hTR-500 (TERC Human)

Expression

Mammalian

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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