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pcDNA3.1 SARS-CoV-2 S D614G

Plasmid

#158075

Purpose

Expresses SARS-CoV-2 spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Neo-PIP-NLS-mVenus

Plasmid

#118619

Purpose

Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-NLS-mVenus

Use

Lentiviral

Tags

mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-NE2h

Plasmid

#208687

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Has Service

AAV5

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-Hsp70-Cas9

Plasmid

#46294

Purpose

A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

codon optimized Cas9

Use

CRISPR

Tags

3x Flag

Expression

Insect

Mutation

Silent change from C to A at bp 834 of insert

Promoter

Hsp70

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Article

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST47-SAR1-GFP

Plasmid

#67409

Purpose

Mammalian expression of hSar1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iCreV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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p4xDRE-Luc

Plasmid

#16534

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

4xDRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

May 12, 2010

Availability

Academic Institutions and Nonprofits only
pmTurquoise2-LAMP1

Plasmid

#110948

Purpose

Expresses LAMP1 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2/103Q

Plasmid

#1385

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (103Q) (HTT Human)

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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