We narrowed to 13,135 results for: lic
-
Plasmid#227582PurposeRelease plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 repliconDepositorInsertCas9
UseCRISPRAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-pheS
Plasmid#111611PurposeYeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.DepositorInsertpheS
ExpressionBacterialAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆FHA-mNeonGreen-IRESpuro2
Plasmid#183739PurposeExpresses Ki67∆FHA-mNeonGreenDepositorInsertMKI67_lacking FHA (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking FHA)PromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
TagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAL1
Plasmid#197285PurposeShuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195DepositorInsertOrigin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
UseSynthetic Biology; Bacterial and yeast cloningAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAurora
Plasmid#190158PurposeRegulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneTagsHis6 tagExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
SLF063: Lenti-fCAG-jRGECO1a
Plasmid#124775PurposeFor general lentiviral or direct expression of cytosolic jRGECO1aDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianPromoterCAGAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSC168
Plasmid#216350PurposexhNup35-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSarQ27G-VMAn-FKBP
Plasmid#70230PurposeExpresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicingDepositorInsertalpha Sarcin (N-terminal portion)
TagsFK506- and rapamycin-binding protein (FKBP), Link…ExpressionMammalianMutationChanged Gln 27 to GlyPromoterCMVAvailable SinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
modified pcAT7-Glo1
Plasmid#160996PurposeSplicing reporter backboneDepositorInserthemoglobin subunit beta (HBB Human)
ExpressionMammalianMutationRepeated intron 1 with 40 nucelotide deletionPromoterCAGAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDG04163
Plasmid#216373PurposexhNup214-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup214-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-scSUMOExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
TagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSC234
Plasmid#216357PurposexhNup93-Nb3t (+2Cys) Nanobody productionDepositorInsertxhNup93-Nb3t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04439
Plasmid#216365PurposexhNup133-Nb2t (+2Cys) Nanobody productionDepositorInsertxhNup133-Nb2t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NGFP-VMAn-FKBP
Plasmid#70225PurposeExpresses N-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
TagsFK506- and rapamycin-binding protein (FKBP), Link…ExpressionMammalianMutationChanged Glu 125 to Ile, Deleted amino acids 129-2…PromoterCMVAvailable SinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FRB-VMAc-CGFP
Plasmid#70226PurposeExpresses C-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
TagsFKBP-rapamycin binding (FRB) domain of the mammal…ExpressionMammalianMutationChanged Ile 129 to Cys, Deleted amino acids 1-128PromoterCMVAvailable SinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTP728
Plasmid#216374PurposexNup358-Nb1t (+3Cys) Nanobody productionDepositorInsertxNup358-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆Nterm-mNeonGreen-IRESpuro2
Plasmid#183740PurposeExpresses Ki67∆Nterm-mNeonGreenDepositorInsertMKI67 LR domain + all Ki-67 repeats (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only