We narrowed to 2,516 results for: organization Addgene

1. TVBB C-term-mTurquoise2-Hygro

   Plasmid

   #169225

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Hygro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TVBB C-term-mTurquoise2-Puro

   Plasmid

   #169224

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SOX2 KO (T2A-H2B-EGFP)

   Plasmid

   #167969

   Purpose

   SOX2 CDS replacement with T2A-H2B-EGFP

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 2 (SOX2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2K-CAGGS-mouse Gfi1-IRES-GFP

   Plasmid

   #91893

   Purpose

   Mammalian expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pPB CAG Cdk6 R31C-IRES-EGFP

   Plasmid

   #153946

   Purpose

   piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R31C

   Promoter

   CAG

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB CAG Gfi1:FLAG-IRES-EGFP

   Plasmid

   #153945

   Purpose

   piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP N1 mitoAURKA

   Plasmid

   #157754

   Purpose

   Expression of AURKA:GFP targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   AURKA without the first 30 amino acids replaced b…

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV ShadowG-AURKA-mTurq2

   Plasmid

   #157768

   Purpose

   Expression of AuroraA kinase biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   mTurquoise2 and shadowG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TVBB C-term-Dendra2-Puro

    Plasmid

    #169217

    Purpose

    Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked Dendra2-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-VRERRA-P2A-GFP-PGK-Puro

    Plasmid

    #110865

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204191

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of R162

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204213

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Point mutation on I207M

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204211

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Point mutation on Q175* (*termination)

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204210

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Point mutation on R178* (*termination)

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204212

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NK2 homeobox 1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Point mutation on I207F

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMeCP2G pc1121

    Plasmid

    #167566

    Purpose

    The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

    Depositor

    Cristina Cardoso

    Article

    Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

    Inserts

    Methyl-CpG binding protein 2 (Mecp2 Rat)
    GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV ShadowY-AURKA-mTurq2

    Plasmid

    #157770

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge