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We narrowed to 31,362 results for: ica

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Showing: 3181 - 3210 of 31362 results

  1. 4A3.PBAD-repL(WT).J23115-gfp

    Plasmid
    #197872
    Purpose
    Arabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expression
    Depositor
    Baojun Wang
    Article
    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.
    Inserts
    part kilA/repL
    gfp
    Expression
    Bacterial
    Mutation
    contains only the 24 bp of 3'-end of kilA ge…
    Promoter
    Bba_J23115 and PBAD
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197872-plasmid-map-sequence-id-395257

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  3. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

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  11. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid
    #202585
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …
    Available Since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    202585-plasmid-map-sequence-id-414431

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  14. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85931-plasmid-map-sequence-id-172473

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  16. lentiMutB3GALT5-blast

    Plasmid
    #208381
    Purpose
    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nucleotide change in PAM targeting sequence nt 51…
    Promoter
    EF-1 alpha core promoter
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208381-plasmid-map-sequence-id-414093

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  24. pT2SCb

    Plasmid
    #59385
    Purpose
    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Insert
    TT-ISceI-cat2
    Use
    Template
    Mutation
    The 3’-end of the chloramphenicol resistance gene…
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59385-plasmid-map-sequence-id-92126

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Showing: 3181 - 3210 of 31362 results