We narrowed to 13,195 results for: 109

1. p21-HA(dPCNA)-NeoR

   Plasmid

   #215108

   Purpose

   Expresses p21-HA(dPCNA) in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR221_SLC29A1

   Plasmid

   #131927

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC29A1 (SLC29A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pHAGE-BRAF-G469A

   Plasmid

   #116153

   Purpose

   Lentiviral expression of BRAF G469A

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G469A

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-BRAF-W531C

   Plasmid

   #116210

   Purpose

   Lentiviral expression of BRAF W531C

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   W531C

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Desmoplakin I acceptor only control (F40-based tension sensor)

   Plasmid

   #119189

   Purpose

   The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-BRAF-K601E

   Plasmid

   #116168

   Purpose

   Lentiviral expression of BRAF K601E

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   K601E

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

   Plasmid

   #159687

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

   Depositor

   Dominic Esposito

   Article

   Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

   Insert

   His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Desmoplakin I affinity mutant (F40-based tension sensor)

   Plasmid

   #119190

   Purpose

   The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

   Plasmid

   #128387

   Purpose

   Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption

   Depositor

   Craig Duvall

   Article

   Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

   Inserts

   NLuc398-3xGGGGS-LGALS8 (LGALS8 Human, Synthetic)
   CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   CLuc394 and NLuc398

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and IRES

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-TetOne-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215147

    Purpose

    Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    TRE3GS

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA4/TO/Strep-HA-ZAKalpha_K45A

    Plasmid

    #141194

    Purpose

    Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    Changed Lysine 45 to Alanine

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-BRAF-D594A

    Plasmid

    #116135

    Purpose

    Lentiviral expression of BRAF D594A

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D594A

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-BRAF-L597R

    Plasmid

    #116178

    Purpose

    Lentiviral expression of BRAF L597R

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L597R

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223_BMPR1B_WT

    Plasmid

    #82195

    Purpose

    Gateway Donor vector containing BMPR1B , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BMPR1B (BMPR1B Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-BRAF-L597S

    Plasmid

    #116179

    Purpose

    Lentiviral expression of BRAF L597S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L597S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid

    #141198

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids and amino acids 6…

    Promoter

    CMV

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-RAF1

    Plasmid

    #116785

    Purpose

    Lentiviral expression of RAF1

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-APTX

    Plasmid

    #215128

    Purpose

    Expresses mCherry-APTX in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    APTX (APTX Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-BRAF-G464V

    Plasmid

    #116148

    Purpose

    Lentiviral expression of BRAF G464V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G464V

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-BRAF-G469E

    Plasmid

    #116154

    Purpose

    Lentiviral expression of BRAF G469E

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G469E

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p21-mCerulean-NeoR

    Plasmid

    #215080

    Purpose

    Expresses p21-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE-BRAF-G466V

    Plasmid

    #116152

    Purpose

    Lentiviral expression of BRAF G466V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G466V

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-BRAF-G464E

    Plasmid

    #116146

    Purpose

    Lentiviral expression of BRAF G464E

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G464E

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-BRAF-L597V

    Plasmid

    #116180

    Purpose

    Lentiviral expression of BRAF L597V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L597V

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CL20_mEGFP_CSF2RA_CRLF2

    Plasmid

    #205798

    Purpose

    Express mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    CSF2RA_CRLF2 (CSF2RA, CRLF2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE-BRAF-G469V

    Plasmid

    #116157

    Purpose

    Lentiviral expression of BRAF G469V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G469V

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE-FAM58A

    Plasmid

    #116739

    Purpose

    Lentiviral expression of FAM58A

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FAM58A (FAM58A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-BRAF-D594N

    Plasmid

    #116138

    Purpose

    Lentiviral expression of BRAF D594N

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D594N

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE-RAF1-S257L

    Plasmid

    #116660

    Purpose

    Lentiviral expression of RAF1 S257L

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S257L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. B52 + CHEK2 sgSTOP

    Plasmid

    #100712

    Purpose

    B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE-BRAF-T599R

    Plasmid

    #116202

    Purpose

    Lentiviral expression of BRAF T599R

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T599R

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE-BRAF-L597Q

    Plasmid

    #116177

    Purpose

    Lentiviral expression of BRAF L597Q

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L597Q

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR223_RAF1_p.L613V

    Plasmid

    #82180

    Purpose

    Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    L613V

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. B52 + FANCM sgSTOP

    Plasmid

    #100710

    Purpose

    B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR223_HIST1H1E_WT

    Plasmid

    #81762

    Purpose

    Gateway Donor vector containing HIST1H1E , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    HIST1H1E (H1-4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHAGE-BRAF-T241P

    Plasmid

    #116197

    Purpose

    Lentiviral expression of BRAF T241P

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T241P

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHAGE-BRAF-T599I

    Plasmid

    #116201

    Purpose

    Lentiviral expression of BRAF T599I

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T599I

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHAGE-BRAF-V600_K601delinsE

    Plasmid

    #116203

    Purpose

    Lentiviral expression of BRAF V600_K601delinsE

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V600_K601delinsE

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mEGFP-N1 YAPS127A- L308/311/325/318E (4LE)

    Plasmid

    #166466

    Purpose

    Expresses fusion of mEGFP and YAPS127A- L308/311/325/318E (4LE)

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAPS127A- L308/311/325/318E (4LE) (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YAPS127A- L308/311/325/318E (4LE)

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHAGE-BRAF-V600M

    Plasmid

    #116208

    Purpose

    Lentiviral expression of BRAF V600M

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V600M

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHAGE-BRAF-T599_V600insP

    Plasmid

    #116199

    Purpose

    Lentiviral expression of BRAF T599_V600insP

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T599_V600insP

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ATX3-77Q-R388G

    Plasmid

    #184251

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
    

    Use

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pPHR-mCherry-hHP1β

    Plasmid

    #103830

    Purpose

    Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-hHP1β (CRY2, CBX1 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pHAGE-BRAF-G466E

    Plasmid

    #116150

    Purpose

    Lentiviral expression of BRAF G466E

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G466E

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHAGE-RAF1-N161K

    Plasmid

    #116656

    Purpose

    Lentiviral expression of RAF1 N161K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N161K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Ankyrin-B scFv [N105/17]

    Plasmid

    #190524

    Purpose

    Mammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/17.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Ankyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Ankyrin-B scFv [N105/13]

    Plasmid

    #190523

    Purpose

    Mammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/13.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Ankyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Anti-Cavbeta1 Ca2+ channel [N7/18.1R]

    Plasmid

    #114497

    Purpose

    Mammalian Expression Plasmid of anti-Cavbeta1 Ca2+ channel (Human). Derived from hybridoma N7/18.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Cavbeta1 Ca2+ channel (Home sapiens) recombinant mouse monoclonal antibody (CACNB1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHAGE-BRAF-K601Q

    Plasmid

    #116170

    Purpose

    Lentiviral expression of BRAF K601Q

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K601Q

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHAGE-BRAF-G469S

    Plasmid

    #116156

    Purpose

    Lentiviral expression of BRAF G469S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G469S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge