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pBAD/HisB-PAiRFP2

Plasmid

#44271

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP2

Expression

Bacterial

Mutation

K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

Promoter

pBAD

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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ERAS

Plasmid

#55656

Purpose

expression of mouse ERAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

ERAS (Eras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
GEM

Plasmid

#55680

Purpose

expression of mouse GEM

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

GEM (Gem Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pLN481 (FuGW-G8p-IL12-1Pe)

Plasmid

#105191

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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fgfprom luc

Plasmid

#69446

Purpose

basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Insert

minimal fgf4 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159851

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pZA27

Plasmid

#158505

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6

Plasmid

#127679

Purpose

Bacterial expression of His-tagged OphA-delta C6

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6

Tags

His8

Expression

Bacterial

Mutation

delta C6

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Plasmid

#159800

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3

Plasmid

#58458

Purpose

Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-hisG

Plasmid

#89954

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

hisG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-H2B-C-10

Plasmid

#55422

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

H2B (H2BC11 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-hygro-eGFP-HEC1-8A/S55D

Plasmid

#114032

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S55D

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)MBII-85-3'ss mut

Plasmid

#67644

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Mutation

3' splice site muattion

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G13-L

Plasmid

#25758

Purpose

Entry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pJL1-aadA

Plasmid

#117050

Purpose

In vitro expression of aadA (streptomycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

aadA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GST

Plasmid

#39286

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag D16A+H588A

Plasmid

#109033

Purpose

D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

Plasmid

#46602

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-24 Decoy (Mir24-1 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pET28b-NmCas9-Flag H588A

Plasmid

#109032

Purpose

H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85854

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-370-5p Sensor (MIR370 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLN491 (FuGW-G8p-CCL21-1Pe)

Plasmid

#105193

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Dectin1A-N-10

Plasmid

#55414

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-pfkB

Plasmid

#102284

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

pfkB gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-xylA

Plasmid

#89964

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

xylA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-lpoB

Plasmid

#89959

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

lpoB gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-His/Vps27(345-440) - Hse(275-375)

Plasmid

#17638

Depositor

James Hurley

Article

Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

Insert

His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pBR322 mCDK4 promoter

Plasmid

#16572

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 promoter (Cdk4 Mouse)

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
mCerulean3-Dectin1A-C-10

Plasmid

#55413

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
myc-actin-NES-1

Plasmid

#60618

Purpose

Encodes MYC tagged beta-actin with a mutation in the NES

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

MYC

Expression

Bacterial and Mammalian

Mutation

I178A, L180A

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
RCAS (B) Eya2 (CT#665)

Plasmid

#13877

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Eya2 (EYA2 Chicken)

Use

Retroviral; Avian expression

Mutation

*

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFIN-IRBP156-tdTOM

Plasmid

#44356

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

IRBP156-tdTOM

Use

Lentiviral

Promoter

murine interphotoreceptor retinoid-binding protei…

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBac-WT-REPAIR

Plasmid

#252296

Purpose

Expresses REPAIR (WT) in insect cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR (WT)

Expression

Insect

Mutation

dPspCas13b H133A/H1058A; ADAR2DD E488Q

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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SpyCas9-T1048-cpGR2(#4670)

Plasmid

#250780

Purpose

Potent chemically-activated and temperature-deactivated SpyCas9

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

SpyCas9 with a cpGR2 insertion behind T1048

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-Dest-uni-gRNA5-BsaI

Plasmid

#241150

Purpose

Destination vector for mini-Golden subcloning platform; contains universal sgRNA at 5' end

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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CreER-pA_FRT-acry-venus

Plasmid

#241166

Purpose

Middle entry vector for mini-Golden subcloning platform; creER; acry-venus

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER; acry-venus

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
P2A-CreER-pA-ORF1-FRT-acry mCherry

Plasmid

#241167

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
P2A-CreER-pA-ORF2-FRT-acry mCherry

Plasmid

#241168

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
P2A-CreER-pA-ORF3-FRT-acry mCherry

Plasmid

#241169

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
PCUP1-GFP-PHO8 K(24,25,30,33)R

Plasmid

#207044

Purpose

Expression of GFP-Pho8 point mutant under CUP1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

GFP

Expression

Yeast

Mutation

K(24,25,30,33)R

Promoter

pCUP1

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
UbSYRAP_pDONR201

Plasmid

#249816

Purpose

Encodes UbSYRAP, a SYRAP split version (UBQ-RAP2.12-GAL4STE12), Gateway compatible as Donor plasmid

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Insert

UBQ-RAP2.12-GAL4STE (RAP2.12 Mustard Weed, Budding Yeast)

Use

Synthetic Biology

Tags

GAL4-STE12 and UBQ

Expression

Plant and Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010-Eco2

Plasmid

#240681

Purpose

RSF1010 origin of replication plasmid containing Eco2 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Eco2 RT, Eco2 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His-Thrombin-Munc13-1 C1-C2B-MUN-C2C (aa529-1735 delta1408-1452 delta1533-1551)

Plasmid

#243821

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO816

Plasmid

#242482

Purpose

Expression of Scatg38 Δ(123 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRECMYC-Eco1

Plasmid

#240705

Purpose

pLAM12 vector backbone containing Eco1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Eco1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4-G1B3

Plasmid

#245067

Purpose

Gateway Entry vector for human G1B3 promoter

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

G1B3 promoter

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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