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We narrowed to 15,565 results for: LEA

Showing: 2121 - 2140 of 15565 results
  1. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Insert
    AMPKsub(TA)-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA-GFP-EBA175-Flag

    Plasmid
    #154970
    Purpose
    Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    GFP and signal peptide (residues 1 to 32) from TG…
    Expression
    Mammalian
    Mutation
    insert codes for EBA175 residues 1284 to 1462
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pRD421

    Plasmid
    #163112
    Purpose
    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS
    Depositor
    Insert
    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    Use
    Tags
    His-tag with TEV-cleavable linker
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. AP4mS_PPVs_P3_cLuc

    Plasmid
    #119300
    Purpose
    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Insert
    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pKS7107

    Plasmid
    #89051
    Purpose
    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
    Depositor
    Inserts
    Nm crRNA
    Nm tracrRNA
    hNmCas9
    Use
    CRISPR
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pExpreS2-1-CR-PA-10H-SIII

    Plasmid
    #175447
    Purpose
    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Type
    Empty backbone
    Use
    Tags
    BiP Secretion Peptide and Thrombin-Protein A-10Hi…
    Expression
    Insect
    Mutation
    Promoter
    Fused Actin-HSP70
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid
    #133085
    Purpose
    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.
    Depositor
    Insert
    Crz1*-CLASP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pADH1
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 2121 - 2140 of 15565 results