We narrowed to 14,015 results for: TIM

1. pAAV-SEP-GluA1 TKIT

   Plasmid

   #169441

   Purpose

   Expression of 2 guides + donor DNA

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   AAV and CRISPR

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

   Plasmid

   #175539

   Purpose

   Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct-Cas9

   Plasmid

   #62209

   Purpose

   Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Expression

   Insect

   Promoter

   act5C

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-yomTagBFP2-SpHis5

   Plasmid

   #44839

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomTagBFP2
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWRS_1001

   Plasmid

   #192205

   Purpose

   lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

   Depositor

   William Sellers

   Article

   Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and RNAi

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR119

   Plasmid

   #13332

   Depositor

   Martin Gellert

   Article

   Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

   Insert

   DNA ligase IV (LIG4 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

7. iRFP713-FRB-Fis1tail

   Plasmid

   #220080

   Purpose

   FRB targeted to the mitochondria with the tail of the mitochondrial outer membrane protein Fis1 with an iRFP fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   FRB-Fis1tail
   

   Expression

   Mammalian

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHT-trxA.ads

   Plasmid

   #47383

   Purpose

   Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Tags

   6xHis and trxA

   Expression

   Bacterial

   Promoter

   Pgrac

   Available Since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJKR-O-mphR

   Plasmid

   #62570

   Purpose

   Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_LAMP1_HaloTag9

    Plasmid

    #175541

    Purpose

    Lyzosomal expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

    Plasmid

    #175540

    Purpose

    Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag9-KDEL
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LT1_36_QUEEN-2m

    Plasmid

    #177708

    Purpose

    Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEF1-QUEEN2m
    

    Use

    Used as donor dna in genome integration after lin…

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV-RabiesG

    Plasmid

    #15785

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))
    

    Expression

    Mammalian

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWRS3001_VCR1_WCR3

    Plasmid

    #192206

    Purpose

    VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone

    Depositor

    William Sellers

    Article

    Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

    Insert

    VCR1-WCR3 tracrRNA
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-nfsB-CFP

    Plasmid

    #86374

    Purpose

    expression of nfsB-CFP

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    nitroreductase
    

    Use

    Zebrafish

    Tags

    ECFP

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_BE4max_3xHA

    Plasmid

    #112096

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_3xHA
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_b4g-HaloTag9

    Plasmid

    #175546

    Purpose

    Expression of HaloTag9 at the Golgi apparatus of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR

    Plasmid

    #235204

    Purpose

    Plasmids for constructing mitoBEs v2

    Depositor

    Wensheng Wei

    Article

    Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR
    

    Expression

    Mammalian

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSETb-pcDronpa2

    Plasmid

    #78184

    Purpose

    Expresses pcDronpa2 in E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    pcDronpa2
    

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

    Plasmid

    #175530

    Purpose

    Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag7-KDEL
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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