We narrowed to 2,367 results for: clu

1. pENTR-BS-AtMIR390a-B/c

   Plasmid

   #199559

   Purpose

   GATEWAY-compatible entry vector

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10

   Insert

   Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAGGS-Flex/3'USS(103)-GFP

   Plasmid

   #197890

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAGGS-Flex/3'USS(553)-GFP

   Plasmid

   #197888

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAGGS-Flex/3'USS(285)-GFP

   Plasmid

   #197889

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. YW347_Ptbb2UTR-loxP-RFP-miniMOS

   Plasmid

   #196064

   Purpose

   a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

   Depositor

   Wenxing Yang

   Article

   Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

   Inserts

   myo2p driven tagRFP
   NeoR
   Minimal Mos1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   myo-2

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

   Plasmid

   #169657

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

   Plasmid

   #169659

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBY011-AMN1ko

   Plasmid

   #183099

   Purpose

   S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.

   Depositor

   Gabor Balazsi

   Article

   Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:

   Insert

   AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
   

   Use

   Tags

   KanMX

   Expression

   Bacterial and Yeast

   Mutation

   only includes external sequences (504 bp each) as…

   Promoter

   URA3, TEF1

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmAGO2-RC_B

   Plasmid

   #145980

   Purpose

   Insect Expression of DmAGO2-RC

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

   Insert

   DmAGO2-RC (AGO2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   A deletion of AA 51-56 and an insertion of 23 AA …

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. VHACg/pDONR221

    Plasmid

    #172094

    Purpose

    Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    530bp upstream of VHA-C ATG

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. GFP-Miro1 (412-618)

    Plasmid

    #127628

    Purpose

    GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    412-618 only

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. UASp-deltaN cycB

    Plasmid

    #112967

    Purpose

    P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Lee et al Genetics. 2001 Aug;158(4):1545-56.

    Insert

    cycB (CycB Fly)
    

    Use

    P insertion vector

    Tags

    Expression

    Mutation

    N-term deletion of first 46 amino acids, includes…

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR CMVie-Lifeact-EGFP L1-R5

    Plasmid

    #84391

    Purpose

    Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1Xpress-AbrR683AN795A-HisC

    Plasmid

    #32510

    Depositor

    Nora Heisterkamp, John Groffen

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    ABR (ABR )
    

    Use

    Tags

    HISC

    Expression

    Mammalian

    Mutation

    R 683 to A and N 795 to A

    Promoter

    Available since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

15. PMmEos3.2

    Plasmid

    #203755

    Purpose

    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Lyn n-term (LYN Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-LBR(1-245)-GFP

    Plasmid

    #222425

    Purpose

    To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR(1-245aa) (LBR Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Truncation (Includes aa 1-245)

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_GR-RE_MLPmin_BC1092-luc2

    Plasmid

    #227112

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-HDR-mEGFP-Actin

    Plasmid

    #119870

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    beta Actin (Actb Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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