We narrowed to 146,089 results for: ins;

1. pMW41: pET-His-MBP-SUMO-R2Bm-SII

   Plasmid

   #209290

   Purpose

   Expresses the R2Bm ORF in E. coli

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2
   

   Use

   Tags

   His, MBP, StrepII, and bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-EGFP-Rab7

   Plasmid

   #174457

   Purpose

   Express EGFP with late endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with late endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab7)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

   Plasmid

   #172067

   Purpose

   Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP11x7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-EGFP-Rab5

   Plasmid

   #174454

   Purpose

   Express EGFP with early endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with early endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab5)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-FKBP-SYNDAPIN2

   Plasmid

   #221311

   Purpose

   Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   SYNDAPIN2 (pLxIS) (Pacsin2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAB076i7

   Plasmid

   #79201

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-β-tubulin-EGFP

   Plasmid

   #175827

   Purpose

   Express EGFP with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with microtuble-targeting sequence
   

   Use

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFX-mCherry-KRasCT

   Plasmid

   #175835

   Purpose

   Express mCherry with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with plasma membrane-targeting sequence
   

   Use

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM6-RgTALsyn

   Plasmid

   #100947

   Purpose

   Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1-alpha-IIb

    Plasmid

    #27288

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha IIb (ITGA2B Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJW6.01

    Plasmid

    #62882

    Purpose

    Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    GFP::MEG-3
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    meg-3

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREDIT_MCP-RecT

    Plasmid

    #164803

    Purpose

    mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Insert

    RecT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-Amp_CRISPIE donor B2

    Plasmid

    #172843

    Purpose

    CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mRuby3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFX-mKate2-Rab11

    Plasmid

    #174463

    Purpose

    Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mKate2 with recycling endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab11)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-mCherry::LANS4

    Plasmid

    #60785

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS4
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-hKCNE1

    Plasmid

    #53050

    Purpose

    Expresses beta subunit for IKs channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-BclXL-pEGFP-C1

    Plasmid

    #177409

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    Bcl-XL
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFX-mito-eqFP650

    Plasmid

    #174179

    Purpose

    Express eqFP650 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    eqFP650 with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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