We narrowed to 14,145 results for: TIM
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pKM5
Plasmid#154166PurposeAedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
TagsFLAGExpressionInsectPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM28
Plasmid#154165PurposeAedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virusDepositorInsertRel1A
TagsFLAGExpressionInsectPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation16 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation12 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM62
Plasmid#154160PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation4 wt NF-κB sites were replaced with Rel2-specific…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM59
Plasmid#154158PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.DepositorInsertFirefly luciferase
UseLuciferasePromoterEnhancer for the Drosophila melanogaster Metchnik…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM52
Plasmid#154155PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM51
Plasmid#154154PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)
Plasmid#133795PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)
Plasmid#133797PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T16A)
TagsNLS(SV40)ExpressionMammalianMutationT16APromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)
Plasmid#133799PurposeCMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Leu-AcrIIA1
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)
Plasmid#133800PurposeCMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Eriv-AcrIIA1
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM143
Plasmid#134166PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM108
Plasmid#134160PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationKMLL, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM107
Plasmid#134159PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq-Tat (c-plasmid)
Plasmid#127635PurposeEncodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.DepositorInsertmTurquoise2
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
DYNLL2
Plasmid#132531PurposeHuman LC8-2, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
DYNLT3
Plasmid#132532PurposeHuman TCTEX-3, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
DYNLRB2
Plasmid#132534PurposeHuman Rbl-2, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 800
Plasmid#131147PurposeTo investigate activity of a 800 bp region upstream of mouse Trib2.DepositorInsert-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 2.6
Plasmid#131151PurposeTo investigate activity of a 2.6 kb region upstream of mouse Trib2.DepositorInsert-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 500
Plasmid#131150PurposeTo investigate activity of a 500 bp region upstream of mouse Trib2.DepositorInsert-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI_MafB_ISH
Plasmid#87670PurposeIn situ probe for mouse MafbDepositorAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-hTtAgo
Plasmid#104383Purposehuman codon optimized TtAgo; pCDNA3 vector backbone, mammalian expressionDepositorInserthTtAgo
Tags3XFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2C pRSETB
Plasmid#104500Purposefluorescent reporter for calcium signalingDepositorInsertTwitch-2C
ExpressionMammalianPromoterCMVAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR11
Plasmid#69154Purposeread-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IVDepositorInsertsUseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterfzr-1 and rps-27Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd96)
Plasmid#177901PurposeBacterial expression of his10-tagged human cd96 Intracellular DomainDepositorInsertCD96 (aa 557-585 only) (CD96 Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)
Plasmid#115141PurposeCMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized Orf2mor anti-CRISPR protein
TagsNLS(SV40)ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianPromotertet-onAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001148862)
Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-CAD
Plasmid#46238PurposeFor expression of HA and FLAG-tagged murine CAD in mammalian cellsDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
TagsFLAG and HAExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianPromotertet-onAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVPromoterU6 and cTnTAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SRRM2-mCherry
Plasmid#174089PurposeInducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on systemDepositorAvailable SinceSept. 12, 2021AvailabilityAcademic Institutions and Nonprofits only
