We narrowed to 18,379 results for: Met

1. Sirius/pRSETB

   Plasmid

   #51956

   Purpose

   bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

   Insert

   Sirius
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. DS-SPcas

   Plasmid

   #48645

   Purpose

   Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   tracrRNA precursor
   

   Use

   CRISPR

   Promoter

   proC and tracrRNA promoter

   Available Since

   Dec. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB1-mCerulean

   Plasmid

   #103968

   Purpose

   Plasmid encoding mCerulean

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mCerulean
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, S65T, …

   Promoter

   ProC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIMP1-Pthl

   Plasmid

   #90485

   Purpose

   Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

   Depositor

   Sheng Yang

   Article

   Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TU#867 (Punc-119sid-1)

   Plasmid

   #25811

   Depositor

   Martin Chalfie

   Article

   Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

   Insert

   systemic RNA interference-deficient 1 (sid-1 Nematode)
   

   Expression

   Worm

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pAH123

   Plasmid

   #66077

   Purpose

   The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   phi80 integrase
   

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD/HisB-iRFP720

   Plasmid

   #45460

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP720
   

   Expression

   Bacterial

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

   Promoter

   araBAD

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-EF1a-MegaGate-HA-Hygro

   Plasmid

   #175268

   Purpose

   MegaGate destination with C-terminal HA tag and hygromycin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBL6

   Plasmid

   #50549

   Purpose

   Expresses mCherry under the PLtetO-1 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-EF1a-MegaGate-HA-Blast

    Plasmid

    #175270

    Purpose

    MegaGate destination with C-terminal HA tag and blasticidin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSET-GCaMP6f

    Plasmid

    #67564

    Purpose

    bacterial expression of a fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    GCaMP6f
    

    Tags

    HIS-tag

    Expression

    Bacterial

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

13. TS245_pNKI-2-13_EGFP-DPN7

    Plasmid

    #200719

    Purpose

    producing Methyl Tracer EGFP protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    eGFP-DPN7
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIP-Vx3K0-mEGFP

    Plasmid

    #35527

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    Vx3K0
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBPHLWL-VP16AD

    Plasmid

    #26259

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    VP16AD-ZIP+
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAL-pck_T

    Plasmid

    #74068

    Purpose

    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (T)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

17. PtxS1-c003

    Plasmid

    #173076

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PtxS1
    

    Expression

    Bacterial

    Mutation

    D35-I221

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mSECFP/pcDNA3

    Plasmid

    #51951

    Purpose

    mammalian expression of mSECFP, a monomeric super enhanced cyan fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

    Insert

    mSECFP
    

    Expression

    Mammalian

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPaxillin-PSmOrange

    Plasmid

    #31923

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Tags

    Paxillin

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. M-ST1cas

    Plasmid

    #48669

    Purpose

    Mammalian S. thermophilus #1 Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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