171,442 results
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Plasmid#204608PurposeMammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-QDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationTadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …Available SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsFLAG tagExpressionMammalianPromoterCAG promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBA439
Plasmid#85967PurposePerturb-seq vector backboneDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-GFP1-10-IRES-Puro
Plasmid#211467PurposeLentivirus backbone expressing GFP1-10DepositorInsertGFP1-10
UseLentiviralExpressionMammalianPromoterEF-1aAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCMV-H2B-FT-Slow
Plasmid#157671PurposeRetroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.DepositorInsertHistone H2B
UseRetroviralTagsSlow-FTExpressionMammalianPromoterCMVAvailable SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-V600E
Plasmid#131723PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOG536
Plasmid#105423PurposeSynthetic biologyDepositorInsertNuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
UseSynthetic BiologyMutationBsaI/ BpiI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CIBN-EGFP-KRasCT
Plasmid#178527PurposeExpresses CIBN-EGFP-KRasCT in mammalian cellsDepositorInsertCIBN-EGFP-KRasCT
ExpressionMammalianPromoterCAGAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-VRQR
Plasmid#159983PurposePrime editing in mammalian cellsDepositorInsertPE2-VRQR
TagsBpSV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
RINS1(mut)
Plasmid#107291PurposePlasmid for the expression of the inactive mutant of RINS1 sensorDepositorInsertRINS1(mut)
TagssfGFPExpressionMammalianMutationtwo Arg/Ser mutations at the beginning and in the…PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only