We narrowed to 6,626 results for: GFP expression plasmids
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Plasmid#225942PurposeLentiviral vector plasmid expressing human ribosome binding protein 1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsmyc-tagExpressionMammalianPromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only
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pHelper-CBE-sgRNA
Plasmid#221137PurposeHelper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with Cas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP6
Plasmid#209968PurposeContains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertionDepositorInsertPromoter Dropout (sfGFP)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PX458-3xHA-en31FnCas9ABEmax8.17d
Plasmid#201956PurposeMammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianPromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en15FnCas9
Plasmid#201948PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en15FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1603H on FnCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT
Plasmid#214075PurposeLuminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
ExpressionMammalianMutationLinker sequence present between firefly luciferas…Available SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
ExpressionMammalianMutationLinker sequence present between firefly luciferas…Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-Emerald-WPRE-UbC-mCherry
Plasmid#211802PurposeLentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoterDepositorInsertsEmerald
mCherry
UseLentiviralExpressionMammalianPromoterSFFV and UbCAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.BB.3z
Plasmid#200675PurposeModular CD8hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald
Plasmid#203803PurposeLentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRV-TdTom-MCS
Plasmid#193711PurposeRetroviral plasmid DNA for subcloning an oligonucleotide library encoding RNA barcodesDepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterCAGAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1670
Plasmid#112044PurposeCEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URADepositorInsertOLE1
TagsV5-TEV-EGFPExpressionYeastMutationP319TPromoterGAL1Available SinceMay 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
Plasmid#149426PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
APEX2 VLP
Plasmid#182211PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingExpressionYeastPromoterGAL1 and GAL10Available SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pELxG4
Plasmid#172570PurposeInducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.DepositorInsertLuxR-GFPmut3
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE-P48R
Plasmid#161816PurposeExpresses ABE-P48R in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCer3-myosinRLC
Plasmid#105759PurposeCyan NMMII phosphorylation sensorDepositorInsertmCer3-RLC
TagsmCer3ExpressionMammalianPromoterCMVAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-395 HA miRFP670[1] IRES miRFP670[2]
Plasmid#130143PurposePPI (BiFC/TriFC) assay vectorDepositorInsertmiRFP670
ExpressionMammalianAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-381 HA UnaG
Plasmid#130132PurposePPI (BiFC/TriFC) assay vectorDepositorInsertUnaG
ExpressionMammalianAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
dps-RFP
Plasmid#63843PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertdps, mRFP1 and GFPmut3b
TagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
katG-RFP
Plasmid#63844PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertkatG, mRFP1 and GFPmut3b
TagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
yqhD-RFP
Plasmid#63845PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertyqhD, mRFP1 and GFPmut3b
TagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a CHR2 mCherry WPRE
Plasmid#129432Purposeexpression of CHR2 mCherry from EF1a promoterDepositorInsertCHR2 mCherry
UseLentiviralAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
nCas9-UGI control for Target-AID (pRZ1047)
Plasmid#131299PurposeCAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).DepositorInsertNLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable SinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT
Plasmid#128551PurposeLentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionMammalianPromoterEF1aAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458-gR2A_ISO
Plasmid#124808PurposeVector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.DepositorInserthSPCas9
Tags3XFLAG and GFPExpressionMammalianAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1673
Plasmid#112047PurposeCEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URADepositorInsertSCT1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-PDX1:VP16
Plasmid#114305PurposeConstitutive expression of a PDX1:VP16 fusion proteinDepositorInsertPDX1-VP16 (Pdx1 Mouse)
UseLentiviralAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
mmu-miR-30a-5p Block-iT design (BLK)
Plasmid#107789PurposemiR-30a RNAi ExpressionDepositorInsertMmu-miR-30a-5p mature sequence (Mir30a Mouse)
UseRNAiTagsEmGFPExpressionMammalianPromoterCMVAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3
Plasmid#107736PurposeIn the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.DepositorInsertsynaptophysin-GCaMP6s, mRuby3
UseAAV and Cre/LoxExpressionMammalianPromoternEFAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p202_LTJ_sgRNACD45.2_R1
Plasmid#82672PurposesgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting mouse CD45.2 region 1
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rab5-L38R-mCherry
Plasmid#102785PurposePoint mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308DDepositorInsertRab5a (RAB5A Human)
TagsmCherryExpressionMammalianMutationLeucine 38 changed to ArgininePromoterCMVAvailable SinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Clover
Plasmid#103991PurposePlasmid encoding CloverDepositorInsertClover
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lazarus Gata 3 flag
Plasmid#80135Purposeretroviral expression of Gata3-FlagDepositorInsertGATA binding protein 3 (Gata3 Mouse)
UseRetroviralTagsFLAG and IRES-EGFPExpressionMammalianAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO MycLAP-STIL ∆CC
Plasmid#80268Purposemammalian expression plasmid for MycLAP-STIL coiled-coil deletionDepositorInsertSTIL (STIL Human)
TagsMyc-GFPExpressionMammalianMutationmissing conserved coiled-coil (amino acids 721-74…PromoterCMVAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2058
Plasmid#49136PurposeAAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.DepositorInsertssAAV-CAGGS-emGFP-WPRE
UseAAVExpressionMammalianPromoterCAGGSAvailable SinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTd-4-15-13-10
Plasmid#63835PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pEMS1177
Plasmid#29091PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1168
Plasmid#29083PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1153
Plasmid#29068PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1111
Plasmid#29040PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1402
Plasmid#29199PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1198
Plasmid#29099PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1135
Plasmid#29055PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1405
Plasmid#29202PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1232
Plasmid#29118PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable SinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCG34
Plasmid#26940DepositorAvailable SinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only