We narrowed to 7,412 results for: CIT

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pAAV hSyn FLEx-FRT hM3Dq-mCherry

Plasmid

#161575

Purpose

Expression of hM3Dq-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM3Dq-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKSJ376

Plasmid

#103065

Purpose

Expresses colicin E1 with R domain deletion and ImmE1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Inserts

colicin E1 immunity protein (pColE1_26, imm E. coli)
cea/colicin E1

Expression

Bacterial

Mutation

R (receptor-binding domain) deletion in colicin E…

Promoter

T7 lac (from pET) and T7 lac (from pET52-b)

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q)

Plasmid

#170125

Purpose

Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD(E488Q)-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB

Plasmid

#223660

Purpose

Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shLacZ

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

Plasmid

#244838

Purpose

Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Article

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3 C163A-His

Plasmid

#11822

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C163A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP2

Plasmid

#71466

Purpose

Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 2 (SENP2 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-SENP2 (catalytic domain)

Plasmid

#16357

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP2 catalytic domain (SENP2 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 365 to 590 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal TDP43 GFP

Plasmid

#27467

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Tags

GFP

Expression

Yeast

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MSP1673

Plasmid

#65769

Purpose

Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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Burden Monitor lambda version

Plasmid

#66073

Purpose

The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
hSirt3-RFP

Plasmid

#216806

Purpose

Human Sirt3-RFP driven by the CaMKII promoter

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (SIRT3 Human)

Use

Lentiviral

Tags

mRFP1

Expression

Mammalian

Promoter

CamKII

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF hM4Di-mCherry

Plasmid

#161579

Purpose

To express hM4Di in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-544

Plasmid

#53548

Purpose

mammalian expression of TPP1 full length

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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