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SHIP2 WT pBABE-His-Xpress

Plasmid

#126584

Purpose

Retroviral infection of MEF cells (puromycin selection)

Depositor

Christophe Erneux

Article

Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

Insert

SHIP2 (INPPL1 Human)

Use

Retroviral

Tags

His Xpress (N-terminal)

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-TetR

Plasmid

#103813

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR (CIB1, tetR E. coli, Mustard Weed)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-delta6xNHL-LIN-41

Plasmid

#52722

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 593–868

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-gfap-sgRNA

Plasmid

#65566

Purpose

in vitro trancription of sgRNA targeting the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap sgRNA (gfap Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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MO70-hJunctate

Plasmid

#79596

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-deltaRING-LIN-41

Plasmid

#52718

Purpose

retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 12-91

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

Plasmid

#69899

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (stw Fly, Human)

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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MO70-hJunctate - EF-mutant

Plasmid

#79597

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

77DAD79 to 77AAA79

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-deltaCoiledCoil-LIN-41

Plasmid

#52720

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 328–447

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57.1 tTA SDHA

Plasmid

#177976

Purpose

All-in-one dox-repressible expression of cDNA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

SDHA (SDHA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Silent point mutations to SDHA sgRNA target

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69894

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-C12AC15A-LIN-41

Plasmid

#52725

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12 and 15 to alanines

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69896

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaBBox-LIN-41

Plasmid

#52719

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 194-320

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaFilamin-LIN-41

Plasmid

#52721

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Filamin domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 483–583

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69886

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SpG_ATH51-IP

Plasmid

#242074

Purpose

Expresses Cas9-SpG_ATH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpG_ATH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf nhsl1b-mNeongreen

Plasmid

#233883

Purpose

mNeongreen tagged form of zebrafish nhsl1b. For in vitro transcription (SP6) or expression through CMV.

Depositor

Nicolas David

Article

Escot et al Commun Biol. 2025 Feb 28;8(1):328. doi:

Insert

nhsl1b (nhsl1b Zebrafish)

Use

In vitro synthesis of mrna

Tags

mNeongreen

Expression

Mammalian

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5c_LxxAA (L3m)

Plasmid

#221441

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5c_LXXAA (L3m) (Fam46c Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Mutation

L332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctr1-gRNA-EGFP

Plasmid

#226000

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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