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pSLHDR02_EGFP_tCD8

Plasmid

#124061

Purpose

Construction of 1st donor for scarless genome editing

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

EGFP
tCD8

Use

Mouse Targeting; Human targeting

Promoter

Human UbC promoter modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-BRD4ΔN-miRFP670-Cry2WT

Plasmid

#122439

Purpose

Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLCE-P2A-Puro

Plasmid

#123318

Purpose

Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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CLYBL hOPM

Plasmid

#112499

Purpose

Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus

Depositor

Bruce Conklin

Insert

OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHLSec2-irisin-his

Plasmid

#122729

Purpose

Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Bovine, Mouse, Human, Rat)

Tags

his and signal sequence for secretion

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTCH1

Plasmid

#116779

Purpose

Lentiviral expression of PTCH1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTCH1 (PTCH1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BAI3

Plasmid

#116716

Purpose

Lentiviral expression of BAI3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BAI3 (BAI3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAC1-P29S

Plasmid

#116650

Purpose

Lentiviral expression of RAC1 P29S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAC1 (RAC1 Human)

Use

Lentiviral

Mutation

P29S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#115344

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV HDAC8_374

Plasmid

#122174

Purpose

Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

Depositor

David Christianson

Article

Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

Insert

His6 MBP TEV HDAC8_374 (HDAC8 Human)

Tags

hexahistidine tag and tobacco etch virus protease…

Expression

Bacterial

Mutation

Residues Met1–Pro7 and H375-V377 have been delete…

Promoter

T7

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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