Skip to main content
Addgene
Showing: 301 - 330 of 1412 results

  9. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

    Enlarge

  10. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60225
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60225-plasmid-map-sequence-id-96391

    Enlarge

  22. pBMN-Z

    Plasmid
    #1734
    Purpose
    This is Nolan lab's standard retrovirus vector backbone that expresses lacZ
    Depositor
    Garry Nolan
    Insert
    LacZ
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 15, 2005
    Availability
    Academic Institutions and Nonprofits only
    1734-plasmid-map-sequence-id-335

    Enlarge

  23. pGfa28-nLac

    Plasmid
    #53130
    Purpose
    Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.
    Depositor
    Michael Brenner
    Article
    Lee et al Glia. 2006 May;53(7):677-87.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains the A and B regions (bp -1757 to -1489) …
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53130-plasmid-map-sequence-id-82730

    Enlarge

  24. pBMN-Z-IN

    Plasmid
    #1735
    Purpose
    This retroviral vector expresses lacZ and has an IRES-NEO downstream
    Depositor
    Garry Nolan
    Insert
    LacZ-IRES-Neo
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2006
    Availability
    Academic Institutions and Nonprofits only
    1735-plasmid-map-sequence-id-336

    Enlarge

Showing: 301 - 330 of 1412 results