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Showing: 501 - 550 of 157662 results

  3. pZA48

    Plasmid
    #158526
    Purpose
    In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1-eGFP
    Use
    Tags
    eGFP
    Expression
    Plant
    Mutation
    L17E, D481V
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158526-plasmid-map-sequence-id-309621

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  5. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  8. pBbB8k-csg-amylase

    Plasmid
    #166859
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    166859-plasmid-map-sequence-id-329647

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  10. pZA8

    Plasmid
    #158486
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158486-plasmid-map-sequence-id-310749

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  13. pPRISM-QF2-he1.1:YFP

    Plasmid
    #184819
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.
    Depositor
    Marnie Halpern
    Article
    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7
    Insert
    QF2-pA-he1.1:YFP
    Use
    CRISPR; Backbone for cloning of homology arms (fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184819-plasmid-map-sequence-id-366121

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  14. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Marnie Halpern
    Article
    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184820-plasmid-map-sequence-id-366119

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  29. pZA16

    Plasmid
    #158494
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, no STOP codon
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    158494-plasmid-map-sequence-id-309733

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  30. pZA7

    Plasmid
    #158485
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158485-plasmid-map-sequence-id-310754

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  31. pZA15

    Plasmid
    #158493
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, no STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158493-plasmid-map-sequence-id-310753

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  34. pZA6

    Plasmid
    #158484
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, STOP codon
    Promoter
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158484-plasmid-map-sequence-id-309731

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  35. pZA14

    Plasmid
    #158492
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, no STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158492-plasmid-map-sequence-id-309703

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  45. pZA28

    Plasmid
    #158506
    Purpose
    In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1-4xMyc (ZAR1 Mustard Weed)
    Use
    Tags
    4xMyc
    Expression
    Plant
    Mutation
    L17E, D489V
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158506-plasmid-map-sequence-id-309636

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  47. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  48. PMXS-SLC1A3

    Plasmid
    #72873
    Purpose
    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    SLC1A3 (SLC1A3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 501 - 550 of 157662 results