We narrowed to 19,752 results for: KIN

1. K84M kinase-dead Nuak1-3xflag

   Plasmid

   #97219

   Purpose

   Plasmid expressing kinase-dead mutant Nuak1

   Depositor

   Huda Zoghbi

   Article

   Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

   Insert

   Nuak1 (NUAK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Kinase-dead K84M

   Promoter

   T7

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. H2B-mScarlet- hMeikin(1-332)-mNeonGreen

   Plasmid

   #174714

   Purpose

   Stable expression of fluorescent Separase cleavage sensor with human Meikin

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin
   

   Use

   Retroviral

   Tags

   H2B-mScarlet and mNeonGreen

   Expression

   Mammalian

   Mutation

   1-332

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Desmoplakin I no force control (F40-based)

   Plasmid

   #119187

   Purpose

   The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   truncation after aa1945

   Promoter

   TCE

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Desmoplakin II tension sensor (F40-based)

   Plasmid

   #118715

   Purpose

   The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-F40-mCherry

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

   Plasmid

   #185682

   Purpose

   Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K599A

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human Inositol 1,4,5-Trisphosphate 3-Kinase B

   Plasmid

   #122589

   Purpose

   Expression of human inositol 1,4,5-trisphosphate 3-kinase B

   Depositor

   Christophe Erneux

   Article

   Dewaste et al Biochem Biophys Res Commun. 2002 Feb 22;

   Insert

   inositol 1,4,5-trisphosphate 3-kinase B (ITPKB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

   Plasmid

   #161586

   Purpose

   expression of Myc-tagged mouse Lrrk2 without kinase domain

   Depositor

   Maik Hintze

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Use

   Tags

   Myc tag and StrepII

   Expression

   Mammalian

   Mutation

   deletion: AA1871-2540

   Promoter

   CMV

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-738pUC19-tNGFR-P2A-Kindlin-3

   Plasmid

   #186092

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   KINDLIN3 (FERMT3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP Kind2 S159/181/666A

   Plasmid

   #105310

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666A

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Desmoplakin I tension sensor (F40-based)

    Plasmid

    #118725

    Purpose

    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Desmoplakin II no force control (F40-based)

    Plasmid

    #118716

    Purpose

    The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1353

    Promoter

    CMV

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pM-ErbB4-delta-kinase-PY3 mutant

    Plasmid

    #17802

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 988-1292, …

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-parkin delta 101-109

    Plasmid

    #227364

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin delta 101-109 (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Resi…

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLv-EF1a-Parkin(K161N)-A92mKO2

    Plasmid

    #213554

    Purpose

    Lentiviral vector for stable expression of K161N Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K161N mutation in Parkin

    Promoter

    EF1a

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLv-EF1a-Parkin(R104A)-A92mKO2

    Plasmid

    #213556

    Purpose

    Lentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R104A mutation in Parkin

    Promoter

    EF1a

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLv-EF1a-Parkin(S65A)-A92mKO2

    Plasmid

    #213557

    Purpose

    Lentiviral vector for stable expression of S65A Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S65A mutation in Parkin

    Promoter

    EF1a

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLv-CMV(trunc)-Parkin-A92mKO2

    Plasmid

    #213549

    Purpose

    Lentiviral vector for stable low expression of Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CMV

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLv-EF1a-Parkin(N273K)-A92mKO2

    Plasmid

    #213555

    Purpose

    Lentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N273K mutation in Parkin

    Promoter

    EF1a

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLv-EF1a-Parkin(W403A)-A92mKO2

    Plasmid

    #213558

    Purpose

    Lentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    W403A mutation in Parkin

    Promoter

    EF1a

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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