Addgene

We narrowed to 8,801 results for: Pol;

Showing: 201 - 220 of 8801 results

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apoeb (apolipoprotein Eb)_L (OZ529)

Plasmid

#27206

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting apoeb (apoeb Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
apoeb (apolipoprotein Eb)_R (OZ530)

Plasmid

#27207

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting apoeb (apoeb Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
p48.unipolyA.dNGFR.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226767

Purpose

vector with NGFR and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ23-NLS

Plasmid

#231891

Purpose

Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q23-NLS (HTT Human)

Use

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Use

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35S SUMO1

Plasmid

#221071

Purpose

Expression of poly H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35S

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly E67R SUMO1

Plasmid

#221072

Purpose

Expression of poly E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

E67R

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35K SUMO1

Plasmid

#221070

Purpose

Expression of poly H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35K

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35R SUMO1

Plasmid

#221069

Purpose

Expression of poly H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35R

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-BiPOLES-mRuby3

Plasmid

#227780

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3

Depositor

Hillel Adesnik

Insert

BiPOLES-mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGGF-bGH polyA-G

Plasmid

#218902

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

bGH polyA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLI

Plasmid

#201440

Purpose

Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJM919_WT His POLI

Plasmid

#201444

Purpose

Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

lacIq

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBAC3-POLY-pnRFP

Plasmid

#201680

Purpose

Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

813

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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RS314-U6(PolII-Lsm)

Plasmid

#200772

Purpose

Pol II expression of U6 Lsm

Depositor

Aaron Hoskins

Article

Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.

Insert

Gal-SNR6-II

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCK005 ubi:mCerulean_ubb-polyA

Plasmid

#195979

Purpose

Expression construct

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubi:mCerulean

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TRE3GV-somBiPOLES-mKate2

Plasmid

#192579

Purpose

AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

TRE3GV

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TRE3GV-somBiPOLES-mCerulean

Plasmid

#192578

Purpose

AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

TRE3GV

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-eGFP

Plasmid

#192583

Purpose

AAV-vector for bidirectional optogenetic manipulation of excitatory neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

CaMKIIa(0.4)

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole4 KO sgRNA

Plasmid

#186935

Purpose

Pole4 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole4 KO sgRNA (Pole4 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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