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Plasmid#80021PurposeMammalian expression of NGFR-KLRFGFKDepositorInsertNGFR (NGFR Human)
UseTagsKLRFGFKExpressionMammalianMutationfused to KLRFGFKPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hysn-flex-dsRed-shscramble
Plasmid#71383PurposeExpresses scramble shRNA Cre-dependently by flex switchDepositorInserthuman synapsin promoter-flex switch-dsRed-shscramble
UseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Puro
Plasmid#135689PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #1
Plasmid#174511PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.DepositorInsert[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro Scr shRNA
Plasmid#30520DepositorInsertScr shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #2
Plasmid#174512PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.DepositorInsertplasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
UseTags3xFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP/RFP-miR-Scrint
Plasmid#19821DepositorInsertScrambled miRNA
UseCre/Lox and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-GFP-shRNA-scramble
Plasmid#182503PurposeExpresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoterDepositorInsertpan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
UseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterhSyn promoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-shSCR
Plasmid#29780DepositorInsertshSCR
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDC-DIO-GFP-shRNA.scramble
Plasmid#184331PurposeExpresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expressionDepositorInserthdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiTagsExpressionMammalianMutationPromoterhistidine decarboxylase promoter fragment that g…Available sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)
Plasmid#160571PurposeVersion of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.DepositorInsertsgRNAscaffold 2.1 scRNA
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) Tac-delta scr
Plasmid#80019PurposeMammalian expression of Tac-delta scrDepositorInsertTac (IL2RA Human)
UseTagsKLRFGFKExpressionMammalianMutationCD25 amino acids 1 to 263 fused to KLRFGFKPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) Myc-tag-Bluescript
Plasmid#8684DepositorInsertHOXB13 (HOXB13 Human)
UseTagsMycExpressionBacterialMutationMyc tag on N-terminalPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)
Plasmid#231361PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA
Plasmid#203451PurposePlasmid expressing active and codon optimised Cas13e with non-targeting gRNADepositorInsertsU6-non targeting (NT) sgRNA
Cas13e
UseAAVTagsHA and NLSExpressionMammalianMutationCodon optimised using GenScript toolPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
SVA-lncRNA AK057321 shRNA 1S scramble
Plasmid#202837PurposeLentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1SDepositorInsertSVA-lncRNA AK057321 shRNA 1S scramble control
UseLentiviralTagsExpressionMammalianMutationPromoter6Available sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SVA-lncRNA AK057321 shRNA 2S scramble
Plasmid#202839PurposeLentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2SDepositorInsertSVA-lncRNA AK057321 shRNA 2S scramble control
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Blast
Plasmid#135688PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNA sCRNA 2.0 (GB2461)
Plasmid#160593PurposeVersion of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.DepositorInsertsgRNA sCRNA 2.0
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK-mPrrn (5-fold decrease transcription)
Plasmid#123478PurposeMoChlo kitDepositorInsertmPrrn (5-fold decrease transcription)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only