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pET28a-mH6-Cas12i2

Plasmid

#120883

Purpose

Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12i2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

H759A

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-dAaCas12b-Act3.0

Plasmid

#158413

Purpose

CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAGT8162 (AATG_dttLbCas12a-i_TTCG)

Plasmid

#192350

Purpose

L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

AATG_dttLbCas12a-i_TTCG

Use

CRISPR

Tags

Expression

Mutation

D156R; E925Q

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huLb5Cas12a

Plasmid

#174684

Purpose

Expresses 6xHis-MBP tagged Lb5Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huLb5Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12c2-noncoding

Plasmid

#120877

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12c2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huHkCas12a

Plasmid

#174683

Purpose

Expresses 6xHis-MBP tagged HkCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huHkCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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LbCas12a-DR-GC-swap-cr in pBCSK+

Plasmid

#126643

Purpose

Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat GC Swap crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-ArCas12a-2AeGFP

Plasmid

#123632

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

ArCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-LpCas12a-2AeGFP

Plasmid

#123635

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

LpCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJCC_058 FnCas12a D917A

Plasmid

#171667

Purpose

For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a D917A

Use

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

changed aspartate 917 to alanine

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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PET28-FnCas12a-EP16

Plasmid

#183636

Purpose

PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

Depositor

Yunzi Luo

Article

Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

Insert

FnCas12a-EP16

Use

CRISPR

Tags

6×His Tag

Expression

Bacterial

Mutation

N607R, K613V, N617R, K180S, K660R, D616N

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-BsCas12a-2AeGFP

Plasmid

#123633

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

BsCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-PrCas12a-2AeGFP

Plasmid

#123636

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

PrCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-BsCas12b-2AeGFP

Plasmid

#121948

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCJH031_proD_LbCas12a-gRNA-GFP_CAM

Plasmid

#183075

Purpose

Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA targeting GFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV

Plasmid

#136380

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV

Use

CRISPR

Tags

Expression

Plant

Mutation

D570A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPcCas12a

Plasmid

#174681

Purpose

Expresses 6xHis-MBP tagged PcCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPcCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge