We narrowed to 5,545 results for: donor

1. Donor_YTHDF2_KO_Puro

   Plasmid

   #186670

   Purpose

   Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Puromycin (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgDonor_lentiGuide

   Plasmid

   #184168

   Purpose

   LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Insert

   sgDonor
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AX53_pS.Donor.S1.TS

   Plasmid

   #100290

   Purpose

   AAVS1-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Expression

   Mammalian

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AV09_pDonor.EP.S1.TS

   Plasmid

   #100297

   Purpose

   AAVS1-targeting expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   PuroR.T2A.EGFP expression unit
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSLBP_mNG_FS_Donor

   Plasmid

   #191387

   Purpose

   Donor for C-terminal tagging of endogenous hSLBP

   Depositor

   Yannick Doyon

   Insert

   mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AY42_pS.Donor.R5

   Plasmid

   #100291

   Purpose

   CCR5-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Expression

   Mammalian

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1Donor_WT_ADRB2_LucReporter

   Plasmid

   #129788

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. EZH2_N_FS_v2_Donor

   Plasmid

   #79901

   Purpose

   N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR and TALEN

   Tags

   3xFLAG_Twin_Strep

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonorTp

   Plasmid

   #211788

   Purpose

   pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR

   Depositor

   Silvia Cardona

   Article

   Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

   Insert

   dhfr
   

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATP1A1_T804N_hPGK1_EBFP_Donor

    Plasmid

    #187455

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N EBFP donor (ATP1A1 Human)
    

    Expression

    Mammalian

    Promoter

    hPGK1

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATM_N_FS_v2_Donor

    Plasmid

    #214815

    Purpose

    N-terminal tagging of human ATM with 3xFLAG_Twin_Strep

    Depositor

    Yannick Doyon

    Insert

    3xFLAG_2xStrep for tagging ATM at the N-terminus (ATM Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG_2xStrep

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AV11_pDonor.EP.S1

    Plasmid

    #100296

    Purpose

    AAVS1-targeting expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    PuroR.T2A.EGFP expression unit
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. AY10_pS.Donor.R5.TS

    Plasmid

    #100292

    Purpose

    CCR5-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRoDonor

    Plasmid

    #178773

    Purpose

    Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    RoCAST left end, cam, RoCAST right end from Rippkaea orientalis
    

    Expression

    Bacterial

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid

    #129794

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G102H

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FancF_N_FS_v2_Donor

    Plasmid

    #79891

    Purpose

    N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG_Twin_Strep

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AL05_pDonor.DMD

    Plasmid

    #100284

    Purpose

    DMD intron 43-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    EGFP expression unit (DMD Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AC62_pDonor.DMD.TS.DR

    Plasmid

    #100288

    Purpose

    DMD intron 43-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    EGFP expression unit (DMD Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLR04_AAVS1_Donor_HLAEB2M

    Plasmid

    #131089

    Purpose

    Donor template to knock in HLA-E fused to B2M

    Depositor

    Xiaojun Lian

    Insert

    HLA-E and B2M with flexible linker
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBsVchDonor

    Plasmid

    #203814

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    Mini-Tn, aprE cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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