We narrowed to 1,270 results for: grna cloning vector

1. CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA

   Plasmid

   #194727

   Purpose

   CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1(D908A) (AAVS1 Acidaminococcus sp.)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173202

   Purpose

   Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_ATP1A1_G7_Dual_sgRNA

   Plasmid

   #173206

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Dual U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

   Plasmid

   #197952

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26 and pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173205

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Dual U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197958

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   CmYLCVp and pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197959

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   2x35S promoter and pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197960

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTBL3241 PB-AtoBpegRNA-mCherry

   Plasmid

   #226666

   Purpose

   PiggyBac cargo vector encoding A to B pegRNA marked with mCherry

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   AtoB pegRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL3401 BtoApegRNA-sfGFP

    Plasmid

    #226667

    Purpose

    PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    BtoA pegRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPR-B_sgRNA-RMP64 (pAVA3500)

    Plasmid

    #239237

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP64
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

    Plasmid

    #239240

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP24
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiCRISPR-B_sgRNA-RMP24 (pAVA3555)

    Plasmid

    #239242

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP24
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiCRISPR-B_sgRNA-RMP24 (pAVA3563)

    Plasmid

    #239258

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP24
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPR-B_sgRNA-RMP64 (pAVA3572)

    Plasmid

    #239259

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP64
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC-U63-tgRNA-Gal80

    Plasmid

    #169030

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFGRNA-LbCpf1-spCas9-MS2

    Plasmid

    #119675

    Purpose

    Empty vector for cloning LbCpf1-spCas9 MS2-fgRNA

    Depositor

    Yongsub Kim

    Article

    Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLL4 sgRNA(MS2)-MS2-hA3a

    Plasmid

    #112130

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. hU6-pegRNA-EF-1α-Cre Template

    Plasmid

    #196036

    Purpose

    The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6 and EF-1-alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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