We narrowed to 611 results for: vp64

1. pHR-PGK-scFv GCN4-sfGFP-VP64

   Plasmid

   #122133

   Purpose

   Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   scFv GCN4-sfGFP-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)

   Plasmid

   #203907

   Purpose

   Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   Gal4LOV(SK22)-VP64-IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEG302 22aa SunTag VP64 gRNA4 (FWA)

   Plasmid

   #120249

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1

   Plasmid

   #202010

   Purpose

   Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   NbGP41P-sfGFP-VP64-GB1
   

   Use

   Synthetic Biology; Moclo level 1 vector

   Tags

   GB1 and sfGFP

   Promoter

   CmYLCV

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

   Plasmid

   #194229

   Purpose

   Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK-LCB1-SynNotch-T2A-mCherry
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

   Plasmid

   #113685

   Purpose

   An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   inverted de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, Cre/Lox, and Synthetic Biology

   Tags

   NLS and VP64

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEG302 5aa SunTag VP64 g4+g17 (FWA)

   Plasmid

   #119672

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEG302 5aa SunTag VP64 gRNA4 (FWA)

   Plasmid

   #115481

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEG302 5aa SunTag VP64 gRNA17 (FWA)

   Plasmid

   #115482

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EW172 CMV-TO-UTRN-14ZF-VP64 (FLP-IN)

    Plasmid

    #236160

    Purpose

    Plasmid encoding the UTRN-14 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    UTRN-14ZF-VP64
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. EW171 CMV-TO-UTRN-43ZF-VP64 (FLP-IN)

    Plasmid

    #236161

    Purpose

    Plasmid encoding the UTRN-43 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    UTRN-43ZF-VP64
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

    Plasmid

    #194230

    Purpose

    Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-LCB3-SynNotch-T2A-mCherry
    

    Use

    Lentiviral

    Mutation

    E45D mutation in LCB3

    Promoter

    PGK

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMOD_D-AtUBI10-NbGP41-sfGFP-VP64-GB1

    Plasmid

    #202012

    Purpose

    Moclo level 1 - Module D, Promoter: AtUBI10, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: pea rbcsE9

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    NbGP41-sfGFP-VP64-GB1
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GB1 and sfGFP

    Promoter

    AtUBI10

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

    Plasmid

    #136912

    Purpose

    DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    pCAG-DmrC-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Chicken Beta Actin

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

    Plasmid

    #160609

    Purpose

    TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2-VP64:Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid

    #120250

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

    Plasmid

    #47457

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Tags

    2A_GFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

    Plasmid

    #194222

    Purpose

    Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    SynNotch-GAL4 DBD-VP64 AD
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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