We narrowed to 4,083 results for: Mcc;

1. pMDC43-FIT2-FLL[157-159]AAA

   Plasmid

   #96994

   Purpose

   Express GFP-tagged mutated mouse FIT2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

   Insert

   MmFIT2 (Fitm2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Amino acid residues 157-159 were mutated (FLL[157…

   Promoter

   35S

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMDC32-FIT2-FLL[157-159]AAA

   Plasmid

   #96991

   Purpose

   Express mouse FIT2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

   Insert

   MmFIT2 (Fitm2 Mouse)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Amino acid residues 157-159 were mutated (FLL[157…

   Promoter

   35S

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. si:ch211-221n23.1_R (OZ604)

   Plasmid

   #35244

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting si:ch211-221n23.1 (si:ch211-221n23.1 Zebrafish)
   

   Use

   Zebrafish targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. si:ch211-221n23.1_L (OZ603)

   Plasmid

   #35243

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting si:ch211-221n23.1 (si:ch211-221n23.1 Zebrafish)
   

   Use

   Zebrafish targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

5. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

   Plasmid

   #159697

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-UbC-Tet1v4-dCas9

   Plasmid

   #232180

   Purpose

   Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGH020 sgp53

   Plasmid

   #227947

   Purpose

   sgRNA targeting p53, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

   Depositor

   Laura Attardi

   Article

   Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

   Insert

   Tp53 (Trp53 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL-CRISPR.EFS.GFP

    Plasmid

    #57818

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EFS and hU6

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4.23-GW

    Plasmid

    #60323

    Purpose

    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-UbC-Tet1c-dCas9

    Plasmid

    #232178

    Purpose

    Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-PGK-EGFP-ALDH1A1-Neo

    Plasmid

    #189742

    Purpose

    The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A1 (ALDH1A1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 TDP-43 NLS1 YFP

    Plasmid

    #84912

    Purpose

    Mammalian expresion of TDP-43 NLS1 YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

    Plasmid

    #159696

    Purpose

    E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
    

    Use

    Tags

    His6-Halotag-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

    Plasmid

    #159698

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227898

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227899

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-AMBRA1-3xFLAG

    Plasmid

    #174159

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227890

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge