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Addgene

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We narrowed to 159,371 results for: addgene

Showing: 2181 - 2200 of 159371 results

  4. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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  5. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  6. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177430-plasmid-map-sequence-id-348458

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  11. pET15b/GVcl 1-1066 /*TBM

    Plasmid
    #162781
    Purpose
    amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Gly 454 to Val
    Promoter
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162781-plasmid-map-sequence-id-321530

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  13. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid
    #117140
    Purpose
    Express gRNA vs Dmap1
    Depositor
    Kosuke Yusa
    Insert
    gDmap1 v1 (Dmap1 Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117140-plasmid-map-sequence-id-233768

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  14. pMAZ.1.0-gDNA

    Plasmid
    #112455
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZ
    Depositor
    Kevin White
    Insert
    MAZ (MAZ Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112455-plasmid-map-sequence-id-230472

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  15. pTHRB.1.0-gDNA

    Plasmid
    #112429
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRB
    Depositor
    Kevin White
    Insert
    THRB (THRB Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112429-plasmid-map-sequence-id-230365

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Showing: 2181 - 2200 of 159371 results