We narrowed to 2,540 results for: organization Addgene

1. pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204214

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of DNA binding domain (delDBD)

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-mouse Ezh2 Y637S-IRES-GFP

   Plasmid

   #91887

   Purpose

   Retroviral expression of mouse Ezh2 with Y637S

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Y637S

   Promoter

   MMLV gag

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

   Plasmid

   #91885

   Purpose

   Retroviral expression of mouse Ezh2 with a SET domain deletion

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   SET domain deleted

   Promoter

   MMLV gag

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-human Erbb2-IRES-GFP

   Plasmid

   #91888

   Purpose

   Retroviral expression of human Erbb2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Erbb2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   MMLV gag

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-WT-WLS-HA-IRES-GFP

   Plasmid

   #178061

   Purpose

   Expression of WLS-HA protein (WT)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

   Plasmid

   #91879

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

7. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

   Plasmid

   #91880

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

8. AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

   Plasmid

   #91884

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Gfi1 (Gfi1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

9. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

   Plasmid

   #91877

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Gfi1 (Gfi1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

10. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

    Plasmid

    #91883

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Gfi1 (Gfi1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pCDH-EF1a-hSNCA

    Plasmid

    #170445

    Purpose

    Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human SNCA (SNCA Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid

    #196196

    Purpose

    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    

    Use

    Homology repair donor

    Tags

    V5-APEX2-mScarlet-I

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #69138

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    

    Tags

    none

    Expression

    Mammalian

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204190

    Purpose

    Doxycycline inducible lentiviral vector for human TFAP2C overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV superYFP-AURKA K162M-mTurq2

    Plasmid

    #157773

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and superYFP

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid

    #222288

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6xHis, GFP, and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

    Plasmid

    #222289

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6His and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV ShadowG-AURKA K162M-mTurq2

    Plasmid

    #157769

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV ShadowY-AURKA K162M-mTurq2

    Plasmid

    #157771

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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