We narrowed to 3,747 results for: mak

1. pLV-Hygro-EF1A-WWE-Linker-eGFP

   Plasmid

   #176072

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   WWE
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Hygro-EF1A-PBZC-Linker-eGFP

   Plasmid

   #176070

   Purpose

   EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PBZ-C
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Hygro-EF1A-PBM-Linker-eGFP

   Plasmid

   #176069

   Purpose

   EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PBM
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mIFP-H2B-C-10

   Plasmid

   #56224

   Purpose

   Localization: Nucleus/Histones, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   H2B (H2BC11 Human)
   

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pcDNA3-MTS-WT-c-Src-FLAG

   Plasmid

   #44652

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Tags

   FLAG tag and mitochondria-targeting sequence (res…

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-DHFR F[3]-N (LEU2)

   Plasmid

   #177798

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-IRES-miR34a-P2

   Plasmid

   #64786

   Purpose

   Luciferase expression driven by miR-34a promoter P2

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Insert

   miR-34a promoter P2 (MIR34A Human)
   

   Use

   Luciferase

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

   Plasmid

   #172514

   Purpose

   Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
   

   Use

   AAV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

   Plasmid

   #183536

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

   Insert

   ChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Mutation

   None

   Promoter

   CaMKIIa

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130997

    Purpose

    Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Promoter

    Ef1a

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-GBF1(E794K)

    Plasmid

    #206416

    Purpose

    Mammalian expression of hGBF1(E794K)

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E794K

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-hChR2(E123A)-EYFP

    Plasmid

    #35505

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSyn1.Twitch2B.WPRE.SV40

    Plasmid

    #100040

    Purpose

    AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Has Service

    AAV1

    Insert

    Twitch2B
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

    Plasmid

    #176542

    Purpose

    Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Tags

    3' extension derived from endogenous locus a…

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS1950

    Plasmid

    #205983

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS1928

    Plasmid

    #205967

    Purpose

    JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH all proteins, with His-tagged Cas8
    

    Tags

    14xHis on Cas8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

    Plasmid

    #176538

    Purpose

    Mammalian expression of nuclear localized AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-WT-VLCAD-FLAG

    Plasmid

    #55823

    Purpose

    Expresses WT-VLCAD in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

    Plasmid

    #61841

    Purpose

    mammalian expression of cyclin B1 non-degradable L45A mutant

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    cyclin B1 (CCNB1 Human)
    

    Tags

    2xSTREP and 3xFLAG

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLVTHM-shRnd3

    Plasmid

    #86437

    Purpose

    2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

    Depositor

    Violaine Moreau

    Article

    Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

    Insert

    shRNA targeting RND3 (RND3 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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