Addgene

We narrowed to 3,518 results for: mak

Showing: 2201 - 2220 of 3518 results

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pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

Plasmid

#39859

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
ABE8.13-d

Plasmid

#136297

Purpose

expresses ABE8.13-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-d

Use

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP12-H2B-C-10

Plasmid

#56254

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Endo-14

Plasmid

#56250

Purpose

Localization: Endosomes, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Endo (RHOB Human)

Use

Tags

Myc and mIFP12

Expression

Mammalian

Mutation

Deleted amino acids 1-4

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
RGS-6xHis-BLMP-1-pcDNA3.1-

Plasmid

#52514

Purpose

expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

blmp-1 (blmp-1 Nematode)

Use

Tags

6xHis

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D RNAiR

Plasmid

#51600

Purpose

expresses RNAi-resistant mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

silent mutations between amino acids (approx 275-…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE-FKBP-mEGFP-WIPI2

Plasmid

#223757

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI2d (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI3

Plasmid

#223763

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI3 (WDR45B Human)

Use

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI3

Plasmid

#223768

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI3 (WDR45B Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI4

Plasmid

#223769

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI4 (WDR45 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d

Plasmid

#223725

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI2d (WIPI2 Human)

Use

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-WIPI4

Plasmid

#223801

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens, James Hurley

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI4 (WDR45 Human)

Use

Tags

GST-TEV

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc RBM42 deletion

Plasmid

#229503

Purpose

Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_Del 363

Use

Tags

Expression

Mammalian

Mutation

Deletion of bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc Compensatory Mutant

Plasmid

#229504

Purpose

Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_CompMutant

Use

Tags

Expression

Mammalian

Mutation

Mutations in bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-EYFP

Plasmid

#231926

Purpose

Cre-on/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-Spo20(PABD)

Plasmid

#221758

Purpose

Stable expression of GFP-Spo20(PABD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Spo20 (SOS1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem Spo20(PABD) (amino acids 422-551)

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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