We narrowed to 54,422 results for: plasmid

1. SIRT6

   Plasmid

   #41565

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SIRT6 (SIRT6 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   aa 3-353

   Promoter

   T7

   Available Since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

2. A-BRDA4-3

   Plasmid

   #166513

   Purpose

   scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 333-460 of 1362, Bromodomain 2

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSlo2.1 Slick/pCDNA3

   Plasmid

   #197317

   Purpose

   Plasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunit

   Depositor

   Christopher Lingle

   Insert

   Slo2.1 (Kcnt2 Mouse)
   

   Tags

   7xHis

   Expression

   Mammalian

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-Syn-H2B-jGCaMP8s-WPRE

   Plasmid

   #176749

   Purpose

   AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   H2B-jGCaMP8s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-dTET1-H1672Y-D1674A-Hygro

   Plasmid

   #104413

   Purpose

   transient expression of dCas9-dTET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dTET1
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   H1672Y-D1674A

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTre-Tight-luciferase-Hottip (522)

   Plasmid

   #65490

   Purpose

   positive control for lncRNA expression assay

   Depositor

   Rory Johnson, Roderic Guigo

   Insert

   Hottip (HOTTIP Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR223 ACE2, open

   Plasmid

   #149719

   Purpose

   For Gateway cloning of ACE2 into destination vector

   Depositor

   Anne-Claude Gingras

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Entry vector

   Mutation

   wt, without stop codon

   Available Since

   June 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. T-TRIM33-4

   Plasmid

   #166550

   Purpose

   scFv of a human scaffold targeting E3 ubiquitin-protein ligase TRIM33. Antigen coverage aa 882-1127 of 1127

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV asyn S129E

   Plasmid

   #36068

   Depositor

   Hilal Lashuel

   Insert

   alpha-synuclein S129E (SNCA Human)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S129E

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

10. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid

    #104418

    Purpose

    transient expression of dCas9-dJMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    dJMJD2A
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    S288G-T289G

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB100

    Plasmid

    #69746

    Purpose

    Retroviral expression of Centrin 3

    Depositor

    Iain Cheeseman

    Insert

    Centrin 3 (CETN3 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

12. mT2A-td-sfGFP-FNF

    Plasmid

    #182339

    Purpose

    Recombineering cassette plasmid for T2A-td-sfGFP-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-td-sfGFP-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.10-Rnd3 promoter [-1759 +248]

    Plasmid

    #86438

    Purpose

    Firefly luciferase reporter under control of the Rnd3 promoter

    Depositor

    Violaine Moreau

    Insert

    Rnd3 promoter (RND3 Human)
    

    Use

    Luciferase

    Tags

    Luciferase (under Rnd3 promoter control)

    Expression

    Mammalian

    Mutation

    promoter sequence derived from HepG2 cells

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Z-FARSB-5

    Plasmid

    #166556

    Purpose

    scFv of a human scaffold targeting phenylalanyl-tRNA synthetase, beta subunit. Antigen coverage aa 1-589 of 589, full-length

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Z-FUS-5

    Plasmid

    #166561

    Purpose

    scFv of a human scaffold targeting RNA-binding protein FUS. Antigen coverage aa 266-526 of 526

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. J-SPRTNC-1

    Plasmid

    #166521

    Purpose

    scFv of a human scaffold targeting SprT-like N-terminal domain. Antigen coverage aa 1-216 of 489

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. ULK3 (ULK3A-c002)

    Plasmid

    #110272

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ULK3 (ULK3 Human)
    

    Tags

    His6-ZBasic-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. G-SP140-8

    Plasmid

    #166520

    Purpose

    scFv of a human scaffold targeting Nuclear body protein SP140. Antigen coverage aa 688-862 of 867, Bromodomain

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

    Plasmid

    #100061

    Purpose

    AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    EF1a

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-GFAP-jGCaMP7c variant 1513

    Plasmid

    #173159

    Purpose

    Expresses jGCaMP7c variant 1513 in astrocytes

    Depositor

    Hervé Chneiweiss, Vincent Vialou

    Insert

    jGCaMP7c variant 1513-WPRE
    

    Use

    AAV

    Promoter

    GFAP

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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