166,034 results

1. dCas9 plasmid

   Plasmid

   #100091

   Purpose

   dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFlag-NLS-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. actc1b-2PH-PLCd-mKate2

   Plasmid

   #109500

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-2PH-PLCd-mKate2
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW

   Plasmid

   #14882

   Depositor

   David Baltimore

   Article

   Lois et al Science. 2002 Feb 1. 295(5556):868-72.

   Insert

   flap-Ub promoter-WRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-CMV 3xFLAG-LC3B-GFP

   Plasmid

   #123107

   Purpose

   Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-PDGFRb-HA

   Plasmid

   #204350

   Purpose

   Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Use

   AAV

   Tags

   HA

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-ISceID44A

   Plasmid

   #59424

   Purpose

   Expresses a catalytically inactive form of the endonuclease ISceI

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   endonuclease ISceI mutant (I-SceI )
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed aspartate 44 to alanine (D44A)

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIC301

   Plasmid

   #69732

   Purpose

   Retroviral expression of Bub1

   Depositor

   Iain Cheeseman

   Insert

   Bub1 (BUB1 Human)
   

   Use

   Retroviral

   Tags

   eGFP-TEV-S

   Expression

   Mammalian

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCAGGS-Akt-FoxO3a-KTR-EGFP

   Plasmid

   #125131

   Purpose

   Fluorescent reporter for Akt activity

   Depositor

   Kazuhiro Aoki

   Article

   Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

   Insert

   FOXO2 (FOXO3 Human)
   

   Tags

   Enhanced green fluorescent protein (EGFP)

   Expression

   Mammalian

   Mutation

   FoxO3A 1-402 amino acid

   Promoter

   CAG promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP C3-YAP2

   Plasmid

   #19055

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScaf-1512.1

    Plasmid

    #111402

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-1512
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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