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lvl0-T [backbone]

Plasmid

#229158

Purpose

Terminator backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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DOX: FSHB-t2a-GFP

Plasmid

#205595

Purpose

an overexpression vector of the killifish FSHB under doxycycline control

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

GFP

Promoter

Ef1a

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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FB376

Plasmid

#209129

Purpose

Multipartite assembly of the TU for YFP expression with the TU for the auxotrophy marker pyr4 from T. reseei

Depositor

Jose Marcos

Article

Ropero-Pérez et al Curr Res Biotechnol 2024

Insert

PgpdA:yfp:TtrpC::Ppyr4:pyr4:Tpyr4

Use

Synthetic Biology

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-CtDCP1_X

Plasmid

#148006

Purpose

Bacterial Expression of CtDCP1

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP1

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Scarlet-I

Plasmid

#193558

Purpose

Expresses mTurquoise2-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-mScarlet-I

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P9-ehaA_LacI

Plasmid

#186317

Purpose

Plasmid for inducible E coli expression of the P9 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P9-ehaA

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP10-ehaA_LacI

Plasmid

#186318

Purpose

Plasmid for inducible E coli expression of the AP10 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP10-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-C2

Plasmid

#155350

Purpose

Express human YTHDF1(360-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (360-559)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4L6

Plasmid

#153157

Purpose

Expression of Flag tagged human FOXD4L6 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4L6 (FOXD4L6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE99

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-C1

Plasmid

#155349

Purpose

Express human YTHDF1(264-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (262-559)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF2-N

Plasmid

#155352

Purpose

Express human YTHDF2(1-381) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF2(1-381)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-N

Plasmid

#155351

Purpose

Express human YTHDF1(1-359) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (1-359)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1C699A

Plasmid

#117245

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1-C699A mutant

Use

Xenopus expression

Mutation

mutated cysteine 699 to alanine

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Cas1

Plasmid

#122285

Purpose

Cas1 cloned in pHMGWA

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas1

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV700

Plasmid

#119017

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV609

Plasmid

#119014

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV688

Plasmid

#119015

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV689

Plasmid

#119016

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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mTalin2_R3

Plasmid

#111035

Purpose

Plasmid expresses the talin2 rod sub domain R3 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3Q->E

Plasmid

#110538

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

O'Rourke et al PLoS One. 2016 Mar 8;11(3):e0150865. doi

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

Q to E mutations in Nab3 low complexity domain

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R2

Plasmid

#111034

Purpose

Plasmid expresses the talin2 rod sub domain R2 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R12

Plasmid

#111045

Purpose

Plasmid expresses the talin2 rod domain R12 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R13

Plasmid

#111046

Purpose

Plasmid expresses the talin2 rod domain R13 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R13-DD

Plasmid

#111047

Purpose

Plasmid expresses the talin2 rod domain R13 and the dimerisation domian DD.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R4

Plasmid

#111036

Purpose

Plasmid expresses the talin2 rod sub domain R4 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R11

Plasmid

#111044

Purpose

Plasmid expresses the talin2 rod domain R11 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSG69-KcsA-Kv1.3-deGFP

Plasmid

#102458

Purpose

Expresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site.

Depositor

Richard Murray

Article

Guo et al bioRxiv 104455

Inserts

KcsA-Kv1.3
deGFP

Tags

His tag

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-38

Plasmid

#79953

Purpose

Myr1-12GS-I3-01-myc-Late4

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-38

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-49

Plasmid

#79964

Purpose

Myr1_G2A-4GS-O3-33-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-49

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-50

Plasmid

#79965

Purpose

Myr1-4GS-O3-33-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-50

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only