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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,358 results for: BLI;

Showing: 4441 - 4480 of 8358 results

  1. m.3047-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211339
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211339-plasmid-map-sequence-id-415656

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  2. m.3075-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211340
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211340-plasmid-map-sequence-id-415644

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  3. m.5147-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211341
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211341-plasmid-map-sequence-id-415657

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  4. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211343
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211343-plasmid-map-sequence-id-415647

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  5. AAV-CAG-K-SPOTIT(R271F)

    Plasmid
    #191492
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT(R271F)
    Use
    AAV
    Tags
    Expression
    Mutation
    R271F in OR
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191492-plasmid-map-sequence-id-377952

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  6. AAV-CAG-M-SPOTIT2(R279F)

    Plasmid
    #191493
    Purpose
    Green fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-SPOTIT2(R279F)
    Use
    AAV
    Tags
    Expression
    Mutation
    R279F in OR
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191493-plasmid-map-sequence-id-377870

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  7. PAS_HeSAHS4

    Plasmid
    #215648
    Purpose
    Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215648-plasmid-map-sequence-id-426093

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  8. PAS_RvSAHS4

    Plasmid
    #215646
    Purpose
    Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215646-plasmid-map-sequence-id-426091

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  9. pX458-tRNA-SpCas9

    Plasmid
    #195183
    Purpose
    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.
    Depositor
    Stephanie Roessler
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    tRNA-Gln
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195183-plasmid-map-sequence-id-423143

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  10. pT7CFE1_EVD68_VP12A_C107R

    Plasmid
    #212982
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A C107R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed cysteine 107 from 2A to Arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212982-plasmid-map-sequence-id-422577

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  11. pT7CFE1_EVD68_3ABC_C147R

    Plasmid
    #212978
    Purpose
    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed Cysteine 147 in 3C to Arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212978-plasmid-map-sequence-id-420358

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  12. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid
    #212980
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed cysteine 147 from 3C to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212980-plasmid-map-sequence-id-420353

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  13. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

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  14. pET28-Flag-RBM24-1~93

    Plasmid
    #208633
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 1~93, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208633-plasmid-map-sequence-id-415921

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  15. pET28-RPL3-Twstrep

    Plasmid
    #208623
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) (RPL3 Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208623-plasmid-map-sequence-id-418316

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  16. pAMW-Wnk

    Plasmid
    #203978
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Use
    Tags
    6xMyc
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203978-plasmid-map-sequence-id-403206

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  17. XBB.1.5+R403K

    Plasmid
    #208586
    Purpose
    Mammalian expression vector for expressing XBB.1.5+R403K SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+R403K spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208586-plasmid-map-sequence-id-409585

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  18. XBB.1.5+F456L

    Plasmid
    #208585
    Purpose
    Mammalian expression vector for expressing XBB.1.5+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+F456L spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208585-plasmid-map-sequence-id-409584

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  19. XBB.1.5+K478R

    Plasmid
    #208587
    Purpose
    Mammalian expression vector for expressing XBB.1.5+K478R SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+K478R spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208587-plasmid-map-sequence-id-409578

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  20. pCDH-Flag-hS1pr1-S5A_Neo

    Plasmid
    #203710
    Purpose
    Overexpression of FLAG-S1PR1-S5A mutant.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-S5A (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S5A
    Promoter
    CMV
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203710-plasmid-map-sequence-id-403867

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  21. pAAV-hPhyB621(Y276H)-P2A-m1V

    Plasmid
    #197377
    Purpose
    The AAV vector for PhyB621(Y276H) and m1V
    Depositor
    Michiyuki Matsuda
    Insert
    Phytochrome B Y276H(1-621 aa)
    Use
    AAV
    Tags
    P2A-m1V
    Expression
    Mutation
    Human codon optimized PhyB (1-621 aa), Y276H muta…
    Promoter
    CMV
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197377-plasmid-map-sequence-id-389799

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  22. pAG416GALL-SARS-CoV-2_3CL

    Plasmid
    #203496
    Purpose
    Expresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    SARS-CoV-2 3CL protease (ORF1ab )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GALL
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203496-plasmid-map-sequence-id-402845

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  23. pAG416GAL10-Bat-CoV-HKU4_3CL

    Plasmid
    #201943
    Purpose
    Expresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    Bat-CoV HKU4 3CL protease (ORF1ab )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201943-plasmid-map-sequence-id-402674

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  24. pALuWBB

    Plasmid
    #183202
    Purpose
    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA
    Depositor
    Mitch McVey
    Insert
    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    Use
    CRISPR; Recombines at attp landing site in fly ge…
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6-3
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    183202-plasmid-map-sequence-id-362157

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  25. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid
    #182894
    Purpose
    Mammalian expression of PVALB rat mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) heavy chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182894-plasmid-map-sequence-id-360571

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  26. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid
    #182895
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182895-plasmid-map-sequence-id-360643

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  27. pLenti-PGK-NXPH4 (neo)

    Plasmid
    #192078
    Purpose
    Lentivirus for expression of NXPH4
    Depositor
    Roland Friedel
    Insert
    NXPH4 (NXPH4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hPGK
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192078-plasmid-map-sequence-id-379235

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  29. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

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  30. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid
    #185966
    Purpose
    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185966-plasmid-map-sequence-id-367346

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  31. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid
    #185965
    Purpose
    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185965-plasmid-map-sequence-id-367345

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  32. pB6-Rosa26-cre_dre_reporter_1_1a

    Plasmid
    #185964
    Purpose
    B6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185964-plasmid-map-sequence-id-367344

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  33. pB6-Rosa26-cre_dre_reporter_1_1

    Plasmid
    #185963
    Purpose
    B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    EGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185963-plasmid-map-sequence-id-367343

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  34. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

    Plasmid
    #182346
    Purpose
    PacI-flanked CAG-LSL expression cassette with a FNF for positive selection
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182346-plasmid-map-sequence-id-358221

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  35. R26-CAG-rox-FNF-bpA-3pA-rox-tdTomato

    Plasmid
    #180154
    Purpose
    tdTomato dre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180154-plasmid-map-sequence-id-355319

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  36. SDCBPA-c021

    Plasmid
    #175485
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175485-plasmid-map-sequence-id-353207

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  37. SDCBPA-c011

    Plasmid
    #175483
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175483-plasmid-map-sequence-id-353170

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  38. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

    Plasmid
    #159919
    Purpose
    Mutagenesis of Vamp1
    Depositor
    Larry Zweifel
    Insert
    Vamp1 (Vamp1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159919-plasmid-map-sequence-id-317336

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  39. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid
    #159907
    Purpose
    Mutagenesis of Netrin1
    Depositor
    Larry Zweifel
    Insert
    Netrin1 (Ntn1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159907-plasmid-map-sequence-id-317313

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  40. pLL1272

    Plasmid
    #129287
    Purpose
    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Araf_1120 as inserts
    Depositor
    Lee Lynd
    Inserts
    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-Araf_1120
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129287-plasmid-map-sequence-id-260451

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Showing: 4441 - 4480 of 8358 results