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pLentiFUCCI(CA)5
Plasmid#223176PurposeLentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)DepositorInsertFUCCI(CA)5
UseLentiviralTagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDualIN-SMN2
Plasmid#214430PurposeDual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7DepositorInsertSMN2 EXON 7 (SMN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PxylA
Plasmid#55178PurposeXylose-inducible promoterDepositorInsertxylA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterxylAAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dat - EGFP - Tol2
Plasmid#85418Purposefor making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) geneDepositorInsertdopamine transporter (slc6a3 Zebrafish)
UseZebrafish transgenesisTagsEGFPExpressionMutationPromoterdatAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB1-A-TelR
Plasmid#204979PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx120-20xMS2-Hyg
Plasmid#210220PurposeIntegrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB3-B-TelR
Plasmid#204992PurposeLevel 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGH42
Plasmid#107249PurposeVipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).DepositorInsertvipp1-gfp
UseSuicide vector for destined for double homologous…TagsmGFPmut3ExpressionBacterialMutationPromoternative promoter of sll0617Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
HypoxCR
Plasmid#59946PurposeA lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.DepositorInsertsGFP-Pest
mCherry-Geminin
UseLentiviralTagsFLAG and PESTExpressionMammalianMutationPromoterCMV and min CMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pλPR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT_tdTomato
Plasmid#138567PurposeUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJDepositorInserttdTomato
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDualEX-SMN2
Plasmid#214431PurposeDual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7DepositorInsertSMN2 EXON 7 (SMN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZJOM75
Plasmid#133649PurposeIntegration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-ANP1-mCherry (ANP1 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
