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pEN_TTmiRc2 3XFLAG-EGFP-NLS

Plasmid

#87778

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

Plasmid

#87777

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7-EE

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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Lf-MetQ

Plasmid

#78305

Purpose

Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

Depositor

Douglas Rees

Article

Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

Insert

Methionine Binding Protein (metQ )

Tags

6x Histidine Tag

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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PU.1-delta75-100 LZRS

Plasmid

#44634

Depositor

Ellen Rothenberg

Article

Dionne et al Dev Biol. 2005 Apr 15;280(2):448-66.

Insert

PU.1 Δ75-100 (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

deletion of aa 75-100

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pNG2470

Plasmid

#37837

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

F13D12.6(G166R) (F13D12.6 Nematode)

Tags

YFP

Expression

Worm

Mutation

G166R, S168G

Promoter

nhx-2

Available Since

Jan. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pNG2462

Plasmid

#37836

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2F13D12.6::YFP (F13D12.6 Nematode)

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-luxAFB-DAS+4

Plasmid

#27064

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxA-FLAG-luxB-DAS+4

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGMCtN-P38-luxAB-DAS+4

Plasmid

#27067

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB-DAS+4 integrative

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pBS TRPS1

Plasmid

#19197

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

TRPS1 (TRPS1 Chicken)

Expression

Bacterial

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only
TM1442-pET-21a

Plasmid

#12650

Depositor

SeWon Suh

Article

Lee et al Proteins. 2004 Jul 1. 56(1):176-9.

Insert

Putative anti-sigma factor antagonist (TM1442 T. maritima)

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pESdelta 799

Plasmid

#13042

Depositor

Peggy Hsieh

Article

Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

Insert

MutS

Tags

His6

Expression

Bacterial

Mutation

C-terminal deletion from aa 800 through 853.

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Tags

GST

Expression

Bacterial

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA2mt

Plasmid

#58218

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA2mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V M339L

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP2-P2A-FusionRed

Plasmid

#188734

Purpose

Express miniGFP2 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Sfp

Plasmid

#159617

Purpose

Expression plasmid for Sfp phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

Sfp

Tags

6HIS

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-mTagBFP2

Plasmid

#216126

Purpose

Lentiviral vector for expressing mTagBFP2.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR2

Plasmid

#236096

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

ExRai-CKAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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c-Flag pcDNA3

Plasmid

#20011

Purpose

This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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